The effect of hepatic fibrosis on T2 value in a mouse model at 3T MRI scanner

• Main Author: Al Sharif, Roaa
• Language: en_US
• Published: Boston University 2018
• Online Access: https://hdl.handle.net/2144/31497

Thesis (M.A.)--Boston University === PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at [e-mail]. Thank you. === PURPOSE: To correlate spin- spin relaxation time (T2) measurements with degree of hepatic fibrosis of ex vivo murine liver specimens at 3T MRI scanner. MATERIALS AND METHODS: The protocol and procedures were approved by The Institutional Animal Care and Use Committee (IACUC). Sixteen male, six week old C578L/6 mice were divided into two groups, a control group (n=1), which was provided normal food, and for liver fibrosis induction an experimental group (n=15), were provided a 0.1% 3,5-diethoxycarbonyl-1 ,4-dihydrocollidine (DOC) to induce liver fibrosis. Exvivo liver specimens were scanned using 3 T MRI scanner. Multi- echo spin-echo sequence was used to perform T2 map. Hematoxylin-eosin with Masson's trichrome staining was performed in liver specimens. Repeated t-test paired sample was used. RESULTS: Increasing the degree of hepatic fibrosis was seen to decrease liver T2 values. CONCLUSION: In this model, liver fibrosis was detected with T2 MR Imaging; the degree of hepatic fibrosis was correlated with the degree of decrease in T2 measurements. === 2031-01-01