L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice

L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice

As we learn more about immune cell subpopulations, we find an increasingly complex system of cells with diverse functions. L2pB1 cells are a PD-L2 positive B1a B lymphocyte subpopulation that has unusual properties and characteristics that are not fully understood by many. By creating and implementi...

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Bibliographic Details
Main Author: Lee, Rebecca Arwyn
Language:en_US
Published: 2016
Subjects:
Immunology
B1a cells
B cells
Depletion
Diabetes
Diphtheria toxin
Transgenic mice
Online Access:https://hdl.handle.net/2144/16050
id ndltd-bu.edu-oai-open.bu.edu-2144-16050
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spelling ndltd-bu.edu-oai-open.bu.edu-2144-160502019-04-06T10:43:28Z L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice Lee, Rebecca Arwyn Immunology B1a cells B cells Depletion Diabetes Diphtheria toxin Transgenic mice As we learn more about immune cell subpopulations, we find an increasingly complex system of cells with diverse functions. L2pB1 cells are a PD-L2 positive B1a B lymphocyte subpopulation that has unusual properties and characteristics that are not fully understood by many. By creating and implementing a transgenic mouse model that allows for targeted depletion of this specific group of cells, we can further elucidate their physiological functions and roles in both healthy and diseased states. Here we demonstrate the depletion of L2pB1 cells utilizing a transgenic mouse model expressing Diphtheria Toxin Receptors on their surface. After a course of 4 injections of 25ng of Diphtheria Toxin per gram bodyweight, we observed a successful depletion of L2pB1 cell population. Further studies are underway investigating the effects of a high fat diet on these L2pB1 depleted mice. 2017-04-01T00:00:00Z 2016-04-22T14:37:43Z 2015 2016-04-08T20:13:24Z Thesis/Dissertation https://hdl.handle.net/2144/16050 en_US Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
collection NDLTD
language en_US
sources NDLTD
topic Immunology
B1a cells
B cells
Depletion
Diabetes
Diphtheria toxin
Transgenic mice
spellingShingle Immunology
B1a cells
B cells
Depletion
Diabetes
Diphtheria toxin
Transgenic mice
Lee, Rebecca Arwyn
L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice
description As we learn more about immune cell subpopulations, we find an increasingly complex system of cells with diverse functions. L2pB1 cells are a PD-L2 positive B1a B lymphocyte subpopulation that has unusual properties and characteristics that are not fully understood by many. By creating and implementing a transgenic mouse model that allows for targeted depletion of this specific group of cells, we can further elucidate their physiological functions and roles in both healthy and diseased states. Here we demonstrate the depletion of L2pB1 cells utilizing a transgenic mouse model expressing Diphtheria Toxin Receptors on their surface. After a course of 4 injections of 25ng of Diphtheria Toxin per gram bodyweight, we observed a successful depletion of L2pB1 cell population. Further studies are underway investigating the effects of a high fat diet on these L2pB1 depleted mice. === 2017-04-01T00:00:00Z
author Lee, Rebecca Arwyn
author_facet Lee, Rebecca Arwyn
author_sort Lee, Rebecca Arwyn
title L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice
title_short L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice
title_full L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice
title_fullStr L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice
title_full_unstemmed L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice
title_sort l2pb1 cell depletion with diphtheria toxin in pd-l2 kiko mice
publishDate 2016
url https://hdl.handle.net/2144/16050
work_keys_str_mv AT leerebeccaarwyn l2pb1celldepletionwithdiphtheriatoxininpdl2kikomice
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