A gene deletion strategy to identify the function of a non-coding RNA in the eukaryotic genome using the model organism Saccharomyces cerevisiae
Non-coding RNA (nc-RNA) plays an important role in biological processes. To understand a non-coding RNA function, we constructed twelve molecular bar-coded deletion mutants in Saccharomyces cerevisiae including five snRNAs, three RNAs of unknown function (RUFs), TLC1, SCR1, NME1 and RPR1. Nine of th...
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University of Manchester
2015
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| Online Access: | https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764394 |