Chemical modifications of graphene for biotechnology applications

• Main Author: Verre, Andrea Francesco
• Other Authors: Vijayaraghavan, Aravind
• Published: University of Manchester 2017
• Subjects: 620; Graphene Adipose Stem Cells Biochemical Functionalization
• Online Access: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.728004

The aim of this thesis is to investigate different functionalization strategy of graphene nanomaterials for graphene-based different biotechnological applications such as graphene-directed stem cell growth and differentiation and graphene-based biosensors. Chemical functionalization of graphene is required in many biological applications; in this thesis we have focused on exploiting the carboxylic groups available on GO molecules and non-covalent functionalization of graphene. GO has been a promising material for stem cell culture due to high specific surface area, ease of functionalization, its ability to support cell proliferation and to not cause cytotoxicity when stem cells are cultured on its substrate. The impact of biochemical functionalization on stem cell differentiation was not widely researched, and many research groups worldwide have been focusing on GO and rGO surfaces only. The approach of this thesis is to fabricate and characterize different graphene-based substrates to investigate the impact of biochemical functionalization of GO in directing adipose stem cell differentiation and to influence the gene expression pathways of Schwann-like differentiated adipose stem cells. The fabrication of graphene based biosensors is still challenging as biological molecules need to be attached to graphene-based sensors to increase both the specificity and the selectivity of the biosensors. In this thesis, two different chemical functionalization approaches were considered. Firstly, the covalent immobilization of membrane proteins embedded on a lipid nanodisc structure on GO was achieved. Secondly, the feasibility of using dip-pen nanolithography as a tool to locally functionalize graphene arrays with phospholipids was demonstrated. Phospholipid interface layer can act as bioactive layer which can be used for the protein insertion of tail-anchoring recombinant proteins as a new route for a non-covalent biological functionalization of graphene array.