The modification of biotin analogues to function as multipurpose labelling tools
This project has focused on establishing a specific multipurpose labelling system via the modification of D-( + )-biotin in use with (strept)avidin proteins for purification and isolation. Two biotin analogues; 2-azidobiotin and 2-propargylbiotin, have been synthesised, introducing an alkyne and azi...
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University of Nottingham
2014
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ndltd-bl.uk-oai-ethos.bl.uk-6643122016-08-04T04:08:50ZThe modification of biotin analogues to function as multipurpose labelling toolsSehmi, Inderpal Kaur2014This project has focused on establishing a specific multipurpose labelling system via the modification of D-( + )-biotin in use with (strept)avidin proteins for purification and isolation. Two biotin analogues; 2-azidobiotin and 2-propargylbiotin, have been synthesised, introducing an alkyne and azide group at the 2 position of the valeryl side chain of biotin respectively. Isothermal titration calorimetry studies showed that the biotin analogues had a reduced affinity (Kd= 1V10-9 M) for strept(avidin) proteins, when compared to biotin (Kd= ~10-15 M).540University of Nottinghamhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.664312Electronic Thesis or Dissertation |
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540 |
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540 Sehmi, Inderpal Kaur The modification of biotin analogues to function as multipurpose labelling tools |
| description |
This project has focused on establishing a specific multipurpose labelling system via the modification of D-( + )-biotin in use with (strept)avidin proteins for purification and isolation. Two biotin analogues; 2-azidobiotin and 2-propargylbiotin, have been synthesised, introducing an alkyne and azide group at the 2 position of the valeryl side chain of biotin respectively. Isothermal titration calorimetry studies showed that the biotin analogues had a reduced affinity (Kd= 1V10-9 M) for strept(avidin) proteins, when compared to biotin (Kd= ~10-15 M). |
| author |
Sehmi, Inderpal Kaur |
| author_facet |
Sehmi, Inderpal Kaur |
| author_sort |
Sehmi, Inderpal Kaur |
| title |
The modification of biotin analogues to function as multipurpose labelling tools |
| title_short |
The modification of biotin analogues to function as multipurpose labelling tools |
| title_full |
The modification of biotin analogues to function as multipurpose labelling tools |
| title_fullStr |
The modification of biotin analogues to function as multipurpose labelling tools |
| title_full_unstemmed |
The modification of biotin analogues to function as multipurpose labelling tools |
| title_sort |
modification of biotin analogues to function as multipurpose labelling tools |
| publisher |
University of Nottingham |
| publishDate |
2014 |
| url |
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.664312 |
| work_keys_str_mv |
AT sehmiinderpalkaur themodificationofbiotinanaloguestofunctionasmultipurposelabellingtools AT sehmiinderpalkaur modificationofbiotinanaloguestofunctionasmultipurposelabellingtools |
| _version_ |
1718373082119274496 |