Kinetics and regulation of the chromaffin granule proton pump

The ATPase from bovine adrenal secretory granules (chromaffin granules) was purified and reconstituted into proteoliposomes. The kinetics of ATP-dependent proton translocation were studied and a fluorimetric assay to allow accurate estimations of initial rates was developed. Rate analysis showed tha...

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Bibliographic Details
Main Author: Webster, Lorna Christina
Published: University of Edinburgh 1996
Subjects:
572
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.663599