Development of the recombinant aequorin method and its evaluation for calcium measurement in filamentous fungi
The aim of this work was to express the Ca2+ sensitive photoprotein, apoaequorin, in filamentous fungi to a high level in order to allow routine and simple measurement of changes in cytosolic Ca2+ ([Ca2+]c) concentration in living hyphae. Analysis of the codon bias of the available apoaequorin genes...
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University of Edinburgh
1999
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.659921 |