Analysis of polar herbicides and steroids by LC-MS using atmospheric pressure ionisation techniques

• Main Author: Browne, Edward R.
• Published: Swansea University 2003
• Subjects: 571.2
• Online Access: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.636164

Chapter one is a brief introduction to solid phase extraction, chromatography and mass spectrometry. A further explanation of the ionisation processes and the interfaces used to allow for coupling of high performance liquid chromatography (HPLC) to mass spectrometry (MS) is provided. The basis of chapter two is the separation and analysis of polar herbicides. HPLC-MS was used to perform analysis of triazine, phenylurea and acidic herbicides. Solid phase extraction was investigated for the analysis of these herbicides in river water and analysis of real samples was conducted. Chapter three involves analysis of free and conjugated estrogen steroids. The analysis of river water extracts spiked with four conjugated estrogen steroids was performed. And solid phase extraction was investigated for the analysis of free and conjugated estrogen steroids in river water and analysis of real river water samples conducted. The development of a method foe the determination of conjugated steroids in male equine urine with HPLC-MS forms the basis of chapter four. The analysis of urine samples taken before and after administration of an anabolic steroid were investigated.