The development and application of multiplex PCR xMAP array assays for the detection of gastrointestinal pathogens in foods and human faecal specimens
Infectious intestinal disease (IID) causes significant morbidity and mortality worldwide. Detection and identification of the causative microorganism is important to allow for effective patient care, outbreak detection and timely public health interventions. which may reduce the number of cases and...
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University of Newcastle Upon Tyne
2010
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.601749 |
| Summary: | Infectious intestinal disease (IID) causes significant morbidity and mortality worldwide. Detection and identification of the causative microorganism is important to allow for effective patient care, outbreak detection and timely public health interventions. which may reduce the number of cases and deaths. Routine detection of bacterial pathogens in foods and faeces relies on culture-based methods and phenotypic identification. However, these methods arc laborious and take several days to achieve results. Also separate methods must be performed for each target pathogen, increasing labour further. PCR-based approaches offer a rapid altemative method. which maintains specific and sensitive detection. |
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