Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model

Abstract Background Living, autologous vascular grafts with the ability to remodel , repair and grow may overcome the limitations of contemporary available options. In this thesis we investigate the ability for autologous, tissue engineered pulmonary artery (PA) conduits to remodel and grow in vivo....

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Main Author:	Cummings, Ian Gustavus
Published:	University of Oxford 2010
Subjects:	616.13
Online Access:	http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.601190

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spelling	ndltd-bl.uk-oai-ethos.bl.uk-6011902015-03-20T06:30:14ZExtracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal modelCummings, Ian Gustavus2010Abstract Background Living, autologous vascular grafts with the ability to remodel , repair and grow may overcome the limitations of contemporary available options. In this thesis we investigate the ability for autologous, tissue engineered pulmonary artery (PA) conduits to remodel and grow in vivo. Methods and Results Vascular grafts fabricated from biodegradable scaffolds were sequentially seeded with autologous vascular derived cells and grown in vitro under biomimetic conditions. These tissue engineered vascular grafts were surgically implanted as main PA replacements in 12 lambs divided into four study groups sacrificed at 20, 50, 80 and 100 weeks post implantation. Transoesophageal echocardiography (TOE) and Computed tomography (CT) angiography was performed in all animals to further assess function and in vivo growth of the tissue engineered PA. Tissue analyses of the conduits were carried out and compared to native tissue over the 2 year study period. The tissue engineered PA showed good functional performance on TOE and CT. There was a significant increase in diameter of the tissue engineered PA of up to 30% and length increases of up to 45%. Histological analysis of tissue engineered PA showed tissue formation comparable to native. Biochemical analysis revealed the cell number and proteoglycan content comparable to native tissue and a significant increase in collagen content over time. The biomechanical analysis showed stronger but less elastic tissue properties compared to native tissue. Matrix metalloproteinases (MMP) as markers for tissue remodelling showed increased active MMP-2 and MMP-9 activity as compared to native tissue. • Conclusion There is on-going remodelling of the tissue engineered PA with increased haemodynamic demands of the growing model.616.13University of Oxfordhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.601190Electronic Thesis or Dissertation
collection	NDLTD
sources	NDLTD
topic	616.13
spellingShingle	616.13 Cummings, Ian Gustavus Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model
description	Abstract Background Living, autologous vascular grafts with the ability to remodel , repair and grow may overcome the limitations of contemporary available options. In this thesis we investigate the ability for autologous, tissue engineered pulmonary artery (PA) conduits to remodel and grow in vivo. Methods and Results Vascular grafts fabricated from biodegradable scaffolds were sequentially seeded with autologous vascular derived cells and grown in vitro under biomimetic conditions. These tissue engineered vascular grafts were surgically implanted as main PA replacements in 12 lambs divided into four study groups sacrificed at 20, 50, 80 and 100 weeks post implantation. Transoesophageal echocardiography (TOE) and Computed tomography (CT) angiography was performed in all animals to further assess function and in vivo growth of the tissue engineered PA. Tissue analyses of the conduits were carried out and compared to native tissue over the 2 year study period. The tissue engineered PA showed good functional performance on TOE and CT. There was a significant increase in diameter of the tissue engineered PA of up to 30% and length increases of up to 45%. Histological analysis of tissue engineered PA showed tissue formation comparable to native. Biochemical analysis revealed the cell number and proteoglycan content comparable to native tissue and a significant increase in collagen content over time. The biomechanical analysis showed stronger but less elastic tissue properties compared to native tissue. Matrix metalloproteinases (MMP) as markers for tissue remodelling showed increased active MMP-2 and MMP-9 activity as compared to native tissue. • Conclusion There is on-going remodelling of the tissue engineered PA with increased haemodynamic demands of the growing model.
author	Cummings, Ian Gustavus
author_facet	Cummings, Ian Gustavus
author_sort	Cummings, Ian Gustavus
title	Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model
title_short	Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model
title_full	Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model
title_fullStr	Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model
title_full_unstemmed	Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model
title_sort	extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model
publisher	University of Oxford
publishDate	2010
url	http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.601190
work_keys_str_mv	AT cummingsiangustavus extracellularmatrixremodellingofautologoustissueengineeredconduitsimplantedinagrowinganimalmodel
_version_	1716797956024172544

Extracellular matrix remodelling of autologous tissue engineered conduits implanted in a growing animal model

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