Stable expression of eukaryotic p450 cDNA in mammalian cell lines

The P450 profile of HepG2 cells was studied using specific enzyme assays and well characterised monoclonal and polyclonal antibodies. These cells were shown to endogenously express cytochrome P450 1A1, P450 1A2 and P450 3A5 when the cells were treated with benz(a)anthracene or rifampicin respectivel...

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Main Author:	Doostdar, Hamed
Published:	University of Aberdeen 1992
Subjects:	571.53
Online Access:	http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.592354

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spelling	ndltd-bl.uk-oai-ethos.bl.uk-5923542017-04-20T03:35:30ZStable expression of eukaryotic p450 cDNA in mammalian cell linesDoostdar, Hamed1992The P450 profile of HepG2 cells was studied using specific enzyme assays and well characterised monoclonal and polyclonal antibodies. These cells were shown to endogenously express cytochrome P450 1A1, P450 1A2 and P450 3A5 when the cells were treated with benz(a)anthracene or rifampicin respectively. HepG2 cells were adapted to grow in Williams' E medium supplemented with only 5% (v/v) fetal calf serum. These cells showed a significantly higher concentration of P450 1A2 protein when treated with benz(a)anthracene and could be detected by P450 1A2 specific antibodies on Western blots. Stable cell lines of HepG2 and Cos-7 cells capable of continuous expression of the Epstein-Barr virus nuclear antigen-1 (EBNA-1) were constructed. P450 2A6 cDNA was successfully expressed in the EBNA-1 transformed Cos-7 cell line for over 6 weeks. The rate of expression of this cDNA and the enzyme activity of the resulting protein was very low. No stable HepG2 EBNA-1 transformed cells capable of P450 2A6 expression was obtained.571.53University of Aberdeenhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.592354http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU602073Electronic Thesis or Dissertation
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sources	NDLTD
topic	571.53
spellingShingle	571.53 Doostdar, Hamed Stable expression of eukaryotic p450 cDNA in mammalian cell lines
description	The P450 profile of HepG2 cells was studied using specific enzyme assays and well characterised monoclonal and polyclonal antibodies. These cells were shown to endogenously express cytochrome P450 1A1, P450 1A2 and P450 3A5 when the cells were treated with benz(a)anthracene or rifampicin respectively. HepG2 cells were adapted to grow in Williams' E medium supplemented with only 5% (v/v) fetal calf serum. These cells showed a significantly higher concentration of P450 1A2 protein when treated with benz(a)anthracene and could be detected by P450 1A2 specific antibodies on Western blots. Stable cell lines of HepG2 and Cos-7 cells capable of continuous expression of the Epstein-Barr virus nuclear antigen-1 (EBNA-1) were constructed. P450 2A6 cDNA was successfully expressed in the EBNA-1 transformed Cos-7 cell line for over 6 weeks. The rate of expression of this cDNA and the enzyme activity of the resulting protein was very low. No stable HepG2 EBNA-1 transformed cells capable of P450 2A6 expression was obtained.
author	Doostdar, Hamed
author_facet	Doostdar, Hamed
author_sort	Doostdar, Hamed
title	Stable expression of eukaryotic p450 cDNA in mammalian cell lines
title_short	Stable expression of eukaryotic p450 cDNA in mammalian cell lines
title_full	Stable expression of eukaryotic p450 cDNA in mammalian cell lines
title_fullStr	Stable expression of eukaryotic p450 cDNA in mammalian cell lines
title_full_unstemmed	Stable expression of eukaryotic p450 cDNA in mammalian cell lines
title_sort	stable expression of eukaryotic p450 cdna in mammalian cell lines
publisher	University of Aberdeen
publishDate	1992
url	http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.592354
work_keys_str_mv	AT doostdarhamed stableexpressionofeukaryoticp450cdnainmammaliancelllines
_version_	1718442214573473792

Stable expression of eukaryotic p450 cDNA in mammalian cell lines

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