Studies into the surface modification of silica-magnetite nanoparticles with ligands for transition metal ion chelation and histidine-tagged protein immobilisation
This project focused on functionalising the surface of silica-magnetite nanoparticles with either acetylated diglycine or terpyridine to produce materials which could bind nickel and be used in the immobilisation/purification of histidine-tagged recombinant protein (specifically protein disulphide i...
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University of Kent
2011
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589965 |
| Summary: | This project focused on functionalising the surface of silica-magnetite nanoparticles with either acetylated diglycine or terpyridine to produce materials which could bind nickel and be used in the immobilisation/purification of histidine-tagged recombinant protein (specifically protein disulphide isomerase). The approach adopted was to 'build the ligands' on the nanoparticle surfaces and involved looking at all aspects of material synthesis, characterisation and application. Three chemical approaches were adopted for material synthesis including 'click' chemistry, carbodiimide mediated coupling of amine and carboxyl groups and the direct interaction of aldehyde with amine-functionalised nanoparticle surfaces. |
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