Combining magnetic tweezers and single-molecule fluorescence microscopy to probe transcription-coupled DNA supercoiling
Previous studies have shown that translocation of an actively transcribing RNA polymerase leads to local increases or decreases in DNA torsion (twin-supercoiled domain), which cannot be resolved in vivo due to interactions of the template DNA, nascent RNA and polymerase with the crowded cellular env...
| Main Author: | |
|---|---|
| Other Authors: | |
| Published: |
University of York
2013
|
| Subjects: | |
| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589316 |