Nanoscale structural and mechanical characterization of fibrin polymerisation
Atomic Force Microscopy (AFM), along with other complementary biophysical techniques, has been used to study several important aspects of the blood clotting process. High resolution imaging modes were used to observe single monomers of fibrinogen, through to oligomers and initial stage proto-fibrils...
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University of Leeds
2009
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505059 |
| Summary: | Atomic Force Microscopy (AFM), along with other complementary biophysical techniques, has been used to study several important aspects of the blood clotting process. High resolution imaging modes were used to observe single monomers of fibrinogen, through to oligomers and initial stage proto-fibrils at sub-molecular resolution in order to understand the molecular basis of fibrin polymerization. Time resolved images of the developing clot were also taken at lower resolution appropriate to the scale of the features. The structures observed were correlated with turbidity measurements to plot the course of the reaction. |
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