Some studies of metal ions in protein systems

The fluorescence interaction of lanthanide ions with a number of amino acids, peptides and proteins has been investigated. Only Tb(III) fluorescence was enhanced by the substances tested. Tb(III) fluorescence was enhanced by tyrosine and peptides containing both tyrosine and tryptophan but not by tr...

Full description

Bibliographic Details
Main Author: Thirkettle, Christine
Published: Loughborough University 1975
Subjects:
543
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.474910
id ndltd-bl.uk-oai-ethos.bl.uk-474910
record_format oai_dc
spelling ndltd-bl.uk-oai-ethos.bl.uk-4749102018-08-07T03:16:13ZSome studies of metal ions in protein systemsThirkettle, Christine1975The fluorescence interaction of lanthanide ions with a number of amino acids, peptides and proteins has been investigated. Only Tb(III) fluorescence was enhanced by the substances tested. Tb(III) fluorescence was enhanced by tyrosine and peptides containing both tyrosine and tryptophan but not by tryptophan alone. The enhancement by tyrosine and the class A protein ribonuclease A was attributed to a collisional interaction, not binding at specific sites. Many class B proteins were shown to enhance Tb(III) fluorescence but the effect was not necessarily associated with vacant metal sites. A detailed study of the fluorescence interaction of Tb(III) with con A and porcine trypsin was made. None of the systems tested gave the huge Tb(III) fluorescence enhancement at small metal:protein ratio shown by transferrin and conalbumin. The interaction in these latter proteins involved rare earth binding at the Fe(III) sites. The interaction in the other class B proteins was probably due to a very complex interaction, possibly involving binding at a number of different sites and collisional interaction.543Loughborough Universityhttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.474910https://dspace.lboro.ac.uk/2134/33853Electronic Thesis or Dissertation
collection NDLTD
sources NDLTD
topic 543
spellingShingle 543
Thirkettle, Christine
Some studies of metal ions in protein systems
description The fluorescence interaction of lanthanide ions with a number of amino acids, peptides and proteins has been investigated. Only Tb(III) fluorescence was enhanced by the substances tested. Tb(III) fluorescence was enhanced by tyrosine and peptides containing both tyrosine and tryptophan but not by tryptophan alone. The enhancement by tyrosine and the class A protein ribonuclease A was attributed to a collisional interaction, not binding at specific sites. Many class B proteins were shown to enhance Tb(III) fluorescence but the effect was not necessarily associated with vacant metal sites. A detailed study of the fluorescence interaction of Tb(III) with con A and porcine trypsin was made. None of the systems tested gave the huge Tb(III) fluorescence enhancement at small metal:protein ratio shown by transferrin and conalbumin. The interaction in these latter proteins involved rare earth binding at the Fe(III) sites. The interaction in the other class B proteins was probably due to a very complex interaction, possibly involving binding at a number of different sites and collisional interaction.
author Thirkettle, Christine
author_facet Thirkettle, Christine
author_sort Thirkettle, Christine
title Some studies of metal ions in protein systems
title_short Some studies of metal ions in protein systems
title_full Some studies of metal ions in protein systems
title_fullStr Some studies of metal ions in protein systems
title_full_unstemmed Some studies of metal ions in protein systems
title_sort some studies of metal ions in protein systems
publisher Loughborough University
publishDate 1975
url http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.474910
work_keys_str_mv AT thirkettlechristine somestudiesofmetalionsinproteinsystems
_version_ 1718718794453483520