Protein engineering and mechanistic studies of morphinone reductase
The thesis describes mechanistic studies of the bacterial protein morphinone reductase (MR) using mutagenesis, kinetic and spectroscopic methods. The roles of seven active site residues were investigated. Cys-191 was initially predicted to be an active site acid; however, mutagenesis studies reveale...
| Main Author: | |
|---|---|
| Published: |
University of Leicester
2004
|
| Subjects: | |
| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409375 |