Fluorescence studies of bovine liver fructose-1.6-diphosphatase

Fluorescence studies of bovine liver fructose-1.6-diphosphatase

Bovine liver fructose-1,6-diphosphatase was purified to homogeneity. Fluorescense studies were used to probe the structure of the active site of the enzyme. Fluorescense probe N-( 1-pyrene) maleimide (NTM) is nonfluorescent in aqueous solution but forms a strong fluorescent adduct with the enzyme th...

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Main Author: Moleli, Lebona E
Format: Others
Published: DigitalCommons@Robert W. Woodruff Library, Atlanta University Center 1988
Online Access:http://digitalcommons.auctr.edu/dissertations/3539
http://digitalcommons.auctr.edu/cgi/viewcontent.cgi?article=5062&context=dissertations
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spelling ndltd-auctr.edu-oai-digitalcommons.auctr.edu-dissertations-50622018-11-07T03:01:51Z Fluorescence studies of bovine liver fructose-1.6-diphosphatase Moleli, Lebona E Bovine liver fructose-1,6-diphosphatase was purified to homogeneity. Fluorescense studies were used to probe the structure of the active site of the enzyme. Fluorescense probe N-( 1-pyrene) maleimide (NTM) is nonfluorescent in aqueous solution but forms a strong fluorescent adduct with the enzyme through its essential sulfhydryl group. The effect of pH, ionic strength, urea, AMP and FDP on environment of the essential sulfhydryl group as measured by the fluorescent probe was studied. 1988-12-01T08:00:00Z text application/pdf http://digitalcommons.auctr.edu/dissertations/3539 http://digitalcommons.auctr.edu/cgi/viewcontent.cgi?article=5062&context=dissertations ETD Collection for AUC Robert W. Woodruff Library DigitalCommons@Robert W. Woodruff Library, Atlanta University Center
collection NDLTD
format Others
sources NDLTD
description Bovine liver fructose-1,6-diphosphatase was purified to homogeneity. Fluorescense studies were used to probe the structure of the active site of the enzyme. Fluorescense probe N-( 1-pyrene) maleimide (NTM) is nonfluorescent in aqueous solution but forms a strong fluorescent adduct with the enzyme through its essential sulfhydryl group. The effect of pH, ionic strength, urea, AMP and FDP on environment of the essential sulfhydryl group as measured by the fluorescent probe was studied.
author Moleli, Lebona E
spellingShingle Moleli, Lebona E
Fluorescence studies of bovine liver fructose-1.6-diphosphatase
author_facet Moleli, Lebona E
author_sort Moleli, Lebona E
title Fluorescence studies of bovine liver fructose-1.6-diphosphatase
title_short Fluorescence studies of bovine liver fructose-1.6-diphosphatase
title_full Fluorescence studies of bovine liver fructose-1.6-diphosphatase
title_fullStr Fluorescence studies of bovine liver fructose-1.6-diphosphatase
title_full_unstemmed Fluorescence studies of bovine liver fructose-1.6-diphosphatase
title_sort fluorescence studies of bovine liver fructose-1.6-diphosphatase
publisher DigitalCommons@Robert W. Woodruff Library, Atlanta University Center
publishDate 1988
url http://digitalcommons.auctr.edu/dissertations/3539
http://digitalcommons.auctr.edu/cgi/viewcontent.cgi?article=5062&context=dissertations
work_keys_str_mv AT molelilebonae fluorescencestudiesofbovineliverfructose16diphosphatase
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