Fluorescence studies of bovine liver fructose-1.6-diphosphatase
Bovine liver fructose-1,6-diphosphatase was purified to homogeneity. Fluorescense studies were used to probe the structure of the active site of the enzyme. Fluorescense probe N-( 1-pyrene) maleimide (NTM) is nonfluorescent in aqueous solution but forms a strong fluorescent adduct with the enzyme th...
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| Format: | Others |
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DigitalCommons@Robert W. Woodruff Library, Atlanta University Center
1988
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| Online Access: | http://digitalcommons.auctr.edu/dissertations/3539 http://digitalcommons.auctr.edu/cgi/viewcontent.cgi?article=5062&context=dissertations |
| Summary: | Bovine liver fructose-1,6-diphosphatase was purified to homogeneity. Fluorescense studies were used to probe the structure of the active site of the enzyme. Fluorescense probe N-( 1-pyrene) maleimide (NTM) is nonfluorescent in aqueous solution but forms a strong fluorescent adduct with the enzyme through its essential sulfhydryl group. The effect of pH, ionic strength, urea, AMP and FDP on environment of the essential sulfhydryl group as measured by the fluorescent probe was studied. |
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