Exploring Techniques to Investigate Mule Deer Diet Composition on the Navajo Nation

• Main Author: Voirin, Chase R.
• Other Authors: Culver, Melanie
• Language: en_US
• Published: The University of Arizona. 2016
• Subjects: Mule Deer; Navajo Nation; Next-Generation Sequencing; Wildife Diet; Natural Resources; Microhistology
• Online Access: http://hdl.handle.net/10150/620710; http://arizona.openrepository.com/arizona/handle/10150/620710

Knowledge of the diet of wildlife can aid wildlife biologists to better understand how a species functions within a given ecosystem. Numerous studies have identified various avenues to examine diet for species throughout the world. Wildlife biologists have used diet composition variables as a means to better understand habitat use and aid in the management and conservation of mule deer, Odocoileus hemionus. The complexity of deer diet is still unknown, and local wildlife management agencies could improve conservation strategies with more information regarding the breadth of plant selection in deer diet. Researchers have used non-invasive methods, such as microhistology via fecal analyses, to assess diet composition for mule deer. However, microhistology has several drawbacks that include accuracy in identification and differentiation of plant species, and even genus, as well as determination of accurate proportions of taxa ingested. Genetic techniques, such as next-generation sequencing (NGS), present new avenues for analyzing herbivore diets, especially through the amplification and analyses of specific regions of chloroplast DNA (cpDNA). Additionally, few studies have directly compared microhistological and NGS diet analyses results for any wildlife species. My objectives were to compare diet composition results of both microhistological and NGS diet analyses through estimating diet richness, taxonomic resolution, percent diet, and frequency of occurrence of plant taxa across samples. Mule deer fecal samples were collected on the Navajo Nation from summer and winter ranges of two distinct mule deer populations, Chuska and Carrizo. I found far greater richness and resolution from NGS of plant taxa through the identification of a greater number of species and genera among all populations, within seasons. Upon testing both methods for both populations, no significant agreement was identified for percent of families identified in the diet with both methods, across all samples. I found trends of positive correlation in the occurrence of families between both methods for Carrizo summer diet, as well as among genera and families in Carrizo winter diet. Upon further statistical analyses, I found no significant positive correlation in the occurrence of genera and families identified with both methods among all samples. Genetic techniques may present innovative methods for determining mule deer diet in various ecosystems, and may also be applied to a broad range of herbivore diet studies.