IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION

IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION

There are many known proteins that are necessary for endocytosis at the plasma membrane. However, little is known about the proteins that are involved in lysosome formation. The Fares lab is interested in identifying novel proteins involved in lysosome formation. A forward genetic screen identified...

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Main Author: Gee, Kevin
Other Authors: Fares, Hanna (Johnny)
Language:en_US
Published: The University of Arizona. 2016
Online Access:http://hdl.handle.net/10150/612977
http://arizona.openrepository.com/arizona/handle/10150/612977
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spelling ndltd-arizona.edu-oai-arizona.openrepository.com-10150-6129772016-06-15T03:05:20Z IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION Gee, Kevin Fares, Hanna (Johnny) There are many known proteins that are necessary for endocytosis at the plasma membrane. However, little is known about the proteins that are involved in lysosome formation. The Fares lab is interested in identifying novel proteins involved in lysosome formation. A forward genetic screen identified cup-12 with a phenotype that indicated a lysosome defect. Using complementation tests and sequencing, I identified cup-12 as clh-6. clh-6 is one of six ClC-type voltage-gated chloride channels in C. elegans. CLH-6 is most closely related to mammalian ClC6 and ClC7. Chloride channels regulate the excitability of cells, volume control, and organelle acidification. Mutations in chloride channels can lead to diseases such as Dent’s disease and Cystic Fibrosis. Our data suggest that loss of some ClCs causes lysosome dysfunction that could contribute to disease symptoms. 2016 text Electronic Thesis http://hdl.handle.net/10150/612977 http://arizona.openrepository.com/arizona/handle/10150/612977 en_US Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. The University of Arizona.
collection NDLTD
language en_US
sources NDLTD
description There are many known proteins that are necessary for endocytosis at the plasma membrane. However, little is known about the proteins that are involved in lysosome formation. The Fares lab is interested in identifying novel proteins involved in lysosome formation. A forward genetic screen identified cup-12 with a phenotype that indicated a lysosome defect. Using complementation tests and sequencing, I identified cup-12 as clh-6. clh-6 is one of six ClC-type voltage-gated chloride channels in C. elegans. CLH-6 is most closely related to mammalian ClC6 and ClC7. Chloride channels regulate the excitability of cells, volume control, and organelle acidification. Mutations in chloride channels can lead to diseases such as Dent’s disease and Cystic Fibrosis. Our data suggest that loss of some ClCs causes lysosome dysfunction that could contribute to disease symptoms.
author2 Fares, Hanna (Johnny)
author_facet Fares, Hanna (Johnny)
Gee, Kevin
author Gee, Kevin
spellingShingle Gee, Kevin
IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION
author_sort Gee, Kevin
title IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION
title_short IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION
title_full IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION
title_fullStr IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION
title_full_unstemmed IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION
title_sort identifying proteins involved in lysosome formation
publisher The University of Arizona.
publishDate 2016
url http://hdl.handle.net/10150/612977
http://arizona.openrepository.com/arizona/handle/10150/612977
work_keys_str_mv AT geekevin identifyingproteinsinvolvedinlysosomeformation
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