Molecular biology of Bunyavirus-host interactions.

Ribonuclease T1 oligonucleotide fingerprint (ONF) analysis was used to study genomic stability of La Crosse virus (Bunyaviridae) during vertical and horizontal transmission in the laboratory. No RNA genomic changes were detected in vertebrate cell culture-propagated virus isolated (following oral in...

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Main Author: Baldridge, Gerald Don.
Other Authors: Hewlett, Martinez J.
Language:en
Published: The University of Arizona. 1989
Subjects:
Online Access:http://hdl.handle.net/10150/184934
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spelling ndltd-arizona.edu-oai-arizona.openrepository.com-10150-1849342015-12-14T03:00:43Z Molecular biology of Bunyavirus-host interactions. Baldridge, Gerald Don. Hewlett, Martinez J. Lindell, Thomas J. Brower, Dannny L. Bernstein, Harris Gerba, Charles P. Bunya viruses Host-virus relationships Virus-vector relationships Viral genetics Amino acid sequence Ribonuclease T1 oligonucleotide fingerprint (ONF) analysis was used to study genomic stability of La Crosse virus (Bunyaviridae) during vertical and horizontal transmission in the laboratory. No RNA genomic changes were detected in vertebrate cell culture-propagated virus isolated (following oral ingestion and replication) from the natural mosquito host, Aedes triseriatus. Genomic changes were not detected during transovarial passage of virus through two generations of mosquitoes or in virus isolated from suckling mice infected by transovarially infected mosquitoes. These results demonstrate that the La Crosse virus genome can remain relatively stable during transovarial transmission (TOT) in the insect host and during transfer between insect and vertebrate hosts. ONF analysis was used to demonstrate TOT of reassortant California serogroup bunyaviruses in Aedes treiseriatus. Mosquitoes were simultaneously inoculated with temperature sensitive mutants of La Crosse and Snowshoe hare viruses able to replicate at 33°C but not at 40°C. Putative reassortants, selected by replication at 40°C, were isolated from progeny mosquitoes and mice infected by these mosquitoes. ONF analysis confirmed that they were reassortants. Approximately 75% of the M segment and 25% of the L segment nucleotide sequences of Inkoo virus (Bunyaviridae) were determined by Sanger dideoxynucleotide sequencing of cDNA clones. Comparison of the M segment nucleotide and deduced amino acid sequences with those of four other bunyaviruses, representing two serogroups, revealed greater conservation of nucleotide than of amino acid sequence between serogroups. Areas of the sequences representing nonstructural protein(s) were less conserved than glycoprotein regions. The L segment nucleotide sequence begins with the known 3' end of the viral L segment and contains an open reading frame encoding the amino terminal 505 amino acids of the viral L protein. The amino acid sequence contains the glycine-aspartate-aspartate motif which is conserved in many RNA-dependent RNA polymerases. Comparison of the L segment sequences with those in the GEN Bank Data Base revealed no significant similarities with any other sequence. 1989 text Dissertation-Reproduction (electronic) http://hdl.handle.net/10150/184934 703608043 9014664 en Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. The University of Arizona.
collection NDLTD
language en
sources NDLTD
topic Bunya viruses
Host-virus relationships
Virus-vector relationships
Viral genetics
Amino acid sequence
spellingShingle Bunya viruses
Host-virus relationships
Virus-vector relationships
Viral genetics
Amino acid sequence
Baldridge, Gerald Don.
Molecular biology of Bunyavirus-host interactions.
description Ribonuclease T1 oligonucleotide fingerprint (ONF) analysis was used to study genomic stability of La Crosse virus (Bunyaviridae) during vertical and horizontal transmission in the laboratory. No RNA genomic changes were detected in vertebrate cell culture-propagated virus isolated (following oral ingestion and replication) from the natural mosquito host, Aedes triseriatus. Genomic changes were not detected during transovarial passage of virus through two generations of mosquitoes or in virus isolated from suckling mice infected by transovarially infected mosquitoes. These results demonstrate that the La Crosse virus genome can remain relatively stable during transovarial transmission (TOT) in the insect host and during transfer between insect and vertebrate hosts. ONF analysis was used to demonstrate TOT of reassortant California serogroup bunyaviruses in Aedes treiseriatus. Mosquitoes were simultaneously inoculated with temperature sensitive mutants of La Crosse and Snowshoe hare viruses able to replicate at 33°C but not at 40°C. Putative reassortants, selected by replication at 40°C, were isolated from progeny mosquitoes and mice infected by these mosquitoes. ONF analysis confirmed that they were reassortants. Approximately 75% of the M segment and 25% of the L segment nucleotide sequences of Inkoo virus (Bunyaviridae) were determined by Sanger dideoxynucleotide sequencing of cDNA clones. Comparison of the M segment nucleotide and deduced amino acid sequences with those of four other bunyaviruses, representing two serogroups, revealed greater conservation of nucleotide than of amino acid sequence between serogroups. Areas of the sequences representing nonstructural protein(s) were less conserved than glycoprotein regions. The L segment nucleotide sequence begins with the known 3' end of the viral L segment and contains an open reading frame encoding the amino terminal 505 amino acids of the viral L protein. The amino acid sequence contains the glycine-aspartate-aspartate motif which is conserved in many RNA-dependent RNA polymerases. Comparison of the L segment sequences with those in the GEN Bank Data Base revealed no significant similarities with any other sequence.
author2 Hewlett, Martinez J.
author_facet Hewlett, Martinez J.
Baldridge, Gerald Don.
author Baldridge, Gerald Don.
author_sort Baldridge, Gerald Don.
title Molecular biology of Bunyavirus-host interactions.
title_short Molecular biology of Bunyavirus-host interactions.
title_full Molecular biology of Bunyavirus-host interactions.
title_fullStr Molecular biology of Bunyavirus-host interactions.
title_full_unstemmed Molecular biology of Bunyavirus-host interactions.
title_sort molecular biology of bunyavirus-host interactions.
publisher The University of Arizona.
publishDate 1989
url http://hdl.handle.net/10150/184934
work_keys_str_mv AT baldridgegeralddon molecularbiologyofbunyavirushostinteractions
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