A Mammalian Virus (SV40) Inductest for Putative Carcinogens

• Main Author: Blount, Howard
• Format: Others
• Published: TopSCHOLAR® 1982
• Subjects: Biology; Diseases; Life Sciences; Medicine and Health Sciences
• Online Access: https://digitalcommons.wku.edu/theses/2140; https://digitalcommons.wku.edu/cgi/viewcontent.cgi?article=3152&context=theses

The in vitro mammalian virus inductest is based on the measurement of induced viral gene expression from Simian virus 40 (SV40) transformed weanling Syrian hamster kidney cells (clone E). Upon challenge by many DNA-damaging chemical agents, infectious virions were produced which were quantitated by assay on a cell line permissive to SV40, such as the highly contact inhibited African green monkey kidney cells, CV-1P. Some known carcinogens and non-carcinogens were tested in this research including mitomycin C, aflatoxins Bl and Gl, sterigmatocystin and several polycyclic hydrocarbons. Lysolecithin (a membrane permeabilizing agent) and S9 mix (a pre -metabolizing mixture) were used in conjunction with the potential carcinogen treatments. Several screening assays for carcinogenic and mutagenic agents are currently in use and the results obtained with these systems were used to determine the reliability of the SV40 inductest system for mammalian cells.