Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment

Combinatorial approaches in metabolic engineering can make use of randomized mutations and/or overexpression of randomized DNA fragments. When DNA fragments are obtained from a common genome or metagenome and packaged into the same expression vector, this is referred to as a DNA library. Generating...

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Bibliographic Details
Main Author: Freedman, Benjamin Gordon
Other Authors: Biological Systems Engineering
Format: Others
Published: Virginia Tech 2016
Subjects:
Online Access:http://hdl.handle.net/10919/71346