Identification of a mutation affecting plasmid establishment in Escherichia coli K-12

A mutation conferring plasmid establishment deficiency in Escherichia coli K-12 strain JF55 has been located on the genetic linkage map. This mutation maps at approximately min 48 and is contransducible with purF. A new use of the process of zygotic induction has been explored to indirectly test the...

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Main Author:	Ferguson, Kaethe Post
Other Authors:	Microbiology
Format:	Others
Language:	en
Published:	Virginia Polytechnic Institute and State University 2016
Subjects:	LD5655.V855 1978.F47
Online Access:	http://hdl.handle.net/10919/64640

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spelling	ndltd-VTETD-oai-vtechworks.lib.vt.edu-10919-646402021-05-05T05:40:15Z Identification of a mutation affecting plasmid establishment in Escherichia coli K-12 Ferguson, Kaethe Post Microbiology LD5655.V855 1978.F47 A mutation conferring plasmid establishment deficiency in Escherichia coli K-12 strain JF55 has been located on the genetic linkage map. This mutation maps at approximately min 48 and is contransducible with purF. A new use of the process of zygotic induction has been explored to indirectly test the ability of an Est⁻ strain to support the first stage in plasmid establishment. According to zygotic induction evidence employing isogenic strains differing only in the est locus mapped, this mutation influences a stage of plasmid establishment later than the conversion of the single-stranded plasmid DNA to the double-stranded form. In addition, evidence is presented that there is more than one gene influencing plasmid establishment in strain JFSS. One such gene is lon. Other mutations, possibly mapping near his and pyrF may influence the first stage of plasmid establishment in this strain. Master of Science 2016-02-01T14:45:12Z 2016-02-01T14:45:12Z 1978 Thesis Text http://hdl.handle.net/10919/64640 en OCLC# 34056763 In Copyright http://rightsstatements.org/vocab/InC/1.0/ v, 78 leaves application/pdf application/pdf Virginia Polytechnic Institute and State University
collection	NDLTD
language	en
format	Others
sources	NDLTD
topic	LD5655.V855 1978.F47
spellingShingle	LD5655.V855 1978.F47 Ferguson, Kaethe Post Identification of a mutation affecting plasmid establishment in Escherichia coli K-12
description	A mutation conferring plasmid establishment deficiency in Escherichia coli K-12 strain JF55 has been located on the genetic linkage map. This mutation maps at approximately min 48 and is contransducible with purF. A new use of the process of zygotic induction has been explored to indirectly test the ability of an Est⁻ strain to support the first stage in plasmid establishment. According to zygotic induction evidence employing isogenic strains differing only in the est locus mapped, this mutation influences a stage of plasmid establishment later than the conversion of the single-stranded plasmid DNA to the double-stranded form. In addition, evidence is presented that there is more than one gene influencing plasmid establishment in strain JFSS. One such gene is lon. Other mutations, possibly mapping near his and pyrF may influence the first stage of plasmid establishment in this strain. === Master of Science
author2	Microbiology
author_facet	Microbiology Ferguson, Kaethe Post
author	Ferguson, Kaethe Post
author_sort	Ferguson, Kaethe Post
title	Identification of a mutation affecting plasmid establishment in Escherichia coli K-12
title_short	Identification of a mutation affecting plasmid establishment in Escherichia coli K-12
title_full	Identification of a mutation affecting plasmid establishment in Escherichia coli K-12
title_fullStr	Identification of a mutation affecting plasmid establishment in Escherichia coli K-12
title_full_unstemmed	Identification of a mutation affecting plasmid establishment in Escherichia coli K-12
title_sort	identification of a mutation affecting plasmid establishment in escherichia coli k-12
publisher	Virginia Polytechnic Institute and State University
publishDate	2016
url	http://hdl.handle.net/10919/64640
work_keys_str_mv	AT fergusonkaethepost identificationofamutationaffectingplasmidestablishmentinescherichiacolik12
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Identification of a mutation affecting plasmid establishment in Escherichia coli K-12

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