Clarification of hog cholera defibrinated blood antitoxin

• Main Author: Henley, Robert R.
• Other Authors: Applied Chemistry
• Format: Others
• Language: en_US
• Published: Virginia Agricultural and Mechanical College and Polytechnic Institute 2015
• Subjects: LD5655.V855 1922.H465; Classical swine fever
• Online Access: http://hdl.handle.net/10919/64066

The factors governing the reaction between chloroform and hemoglobin by which the hemoglobin of the blood may be precipitated were studied, and a process, based upon this reaction, for the separation of a clear sterile serum from old defibrinated blood antitoxin was devised. It is shown that the yield of clear serum separated by this process approximates 70% of the original volume and that the product of the process is free from bacterial contamination. While it is shown by analysis that the globulin content of the serum suffers a slight loss, when the serum is treated by this process, potency tests indicate that the loss in antibodies that occurs during clarification may be disregarded. === Master of Science