Clarification of hog cholera defibrinated blood antitoxin

The factors governing the reaction between chloroform and hemoglobin by which the hemoglobin of the blood may be precipitated were studied, and a process, based upon this reaction, for the separation of a clear sterile serum from old defibrinated blood antitoxin was devised. It is shown that the...

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Bibliographic Details
Main Author: Henley, Robert R.
Other Authors: Applied Chemistry
Format: Others
Language:en_US
Published: Virginia Agricultural and Mechanical College and Polytechnic Institute 2015
Subjects:
Online Access:http://hdl.handle.net/10919/64066
Description
Summary:The factors governing the reaction between chloroform and hemoglobin by which the hemoglobin of the blood may be precipitated were studied, and a process, based upon this reaction, for the separation of a clear sterile serum from old defibrinated blood antitoxin was devised. It is shown that the yield of clear serum separated by this process approximates 70% of the original volume and that the product of the process is free from bacterial contamination. While it is shown by analysis that the globulin content of the serum suffers a slight loss, when the serum is treated by this process, potency tests indicate that the loss in antibodies that occurs during clarification may be disregarded. === Master of Science