Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)
Alterations of immune parameters were investigated in fish exposed to non-overtly toxic levels of three different environmentally relevant chemicals, 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8- tetrachlorodibenzo-p -dioxin (TCDD). Each chemical agent was adminis...
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ndltd-VTETD-oai-vtechworks.lib.vt.edu-10919-448092021-06-22T05:29:20Z Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) Hart, Laura J. Veterinary Medical Sciences Holladay, Steven D. Smith, Bonnie J. Smith, Stephen A. Nagarkatti, Mitzi flow cytometry histology immunosuppression fish hematopoietic organs macrophage LD5655.V855 1996.H378 Alterations of immune parameters were investigated in fish exposed to non-overtly toxic levels of three different environmentally relevant chemicals, 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8- tetrachlorodibenzo-p -dioxin (TCDD). Each chemical agent was administered to tilapia in separate experiments by intraperitoneal injection for five consecutive days. Following the final dose, total cellularity and histology of the spleen and pronephros were assessed, as were activity of phagocytic celis contained in these hematopoietic organs. <p>Using chemical doses which produced no clinical toxicity, tilapia exposed to each chemical agent displayed a significant reduction in total cell number of both spleen and pronephros, in most cases in a dose-related manner. Consistent with this observation, splenic and pronephric hypocellularity was confirmed upon histological examination of chemical-treated fish. However, neither superoxide radical production or phagocytosis of splenic or pronephric macrophages was inhibited in either DMBA, lindane, or TCDD exposed fish. Results of this study indicate that depressed total cell number in fish hematopoietic organs may be a more sensitive indicator of exposure to these environmental contaminants than is the activity of macrophages contained within these organs. Master of Science 2014-03-14T21:45:59Z 2014-03-14T21:45:59Z 1996-07-15 2008-09-18 2008-09-18 2008-09-18 Thesis Text etd-09182008-063320 http://hdl.handle.net/10919/44809 http://scholar.lib.vt.edu/theses/available/etd-09182008-063320/ en OCLC# 35652800 LD5655.V855_1996.H378.pdf In Copyright http://rightsstatements.org/vocab/InC/1.0/ ix, 138 leaves BTD application/pdf application/pdf Virginia Tech |
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flow cytometry histology immunosuppression fish hematopoietic organs macrophage LD5655.V855 1996.H378 |
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flow cytometry histology immunosuppression fish hematopoietic organs macrophage LD5655.V855 1996.H378 Hart, Laura J. Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) |
description |
Alterations of immune parameters were investigated in fish
exposed to non-overtly toxic levels of three different
environmentally relevant chemicals, 7,12-dimethylbenzanthracene
(DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-
tetrachlorodibenzo-p -dioxin (TCDD). Each chemical agent was
administered to tilapia in separate experiments by intraperitoneal
injection for five consecutive days. Following the final dose, total
cellularity and histology of the spleen and pronephros were
assessed, as were activity of phagocytic celis contained in these
hematopoietic organs.
<p>Using chemical doses which produced no clinical toxicity, tilapia
exposed to each chemical agent displayed a significant reduction in
total cell number of both spleen and pronephros, in most cases in a
dose-related manner. Consistent with this observation, splenic and
pronephric hypocellularity was confirmed upon histological
examination of chemical-treated fish. However, neither superoxide
radical production or phagocytosis of splenic or pronephric
macrophages was inhibited in either DMBA, lindane, or TCDD exposed
fish. Results of this study indicate that depressed total cell number
in fish hematopoietic organs may be a more sensitive indicator of
exposure to these environmental contaminants than is the activity
of macrophages contained within these organs. === Master of Science |
author2 |
Veterinary Medical Sciences |
author_facet |
Veterinary Medical Sciences Hart, Laura J. |
author |
Hart, Laura J. |
author_sort |
Hart, Laura J. |
title |
Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) |
title_short |
Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) |
title_full |
Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) |
title_fullStr |
Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) |
title_full_unstemmed |
Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) |
title_sort |
subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (dmba), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>oreochromis niloticus</i>) |
publisher |
Virginia Tech |
publishDate |
2014 |
url |
http://hdl.handle.net/10919/44809 http://scholar.lib.vt.edu/theses/available/etd-09182008-063320/ |
work_keys_str_mv |
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