Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)

Alterations of immune parameters were investigated in fish exposed to non-overtly toxic levels of three different environmentally relevant chemicals, 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8- tetrachlorodibenzo-p -dioxin (TCDD). Each chemical agent was adminis...

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Main Author: Hart, Laura J.
Other Authors: Veterinary Medical Sciences
Format: Others
Language:en
Published: Virginia Tech 2014
Subjects:
Online Access:http://hdl.handle.net/10919/44809
http://scholar.lib.vt.edu/theses/available/etd-09182008-063320/
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spelling ndltd-VTETD-oai-vtechworks.lib.vt.edu-10919-448092021-06-22T05:29:20Z Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>) Hart, Laura J. Veterinary Medical Sciences Holladay, Steven D. Smith, Bonnie J. Smith, Stephen A. Nagarkatti, Mitzi flow cytometry histology immunosuppression fish hematopoietic organs macrophage LD5655.V855 1996.H378 Alterations of immune parameters were investigated in fish exposed to non-overtly toxic levels of three different environmentally relevant chemicals, 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8- tetrachlorodibenzo-p -dioxin (TCDD). Each chemical agent was administered to tilapia in separate experiments by intraperitoneal injection for five consecutive days. Following the final dose, total cellularity and histology of the spleen and pronephros were assessed, as were activity of phagocytic celis contained in these hematopoietic organs. <p>Using chemical doses which produced no clinical toxicity, tilapia exposed to each chemical agent displayed a significant reduction in total cell number of both spleen and pronephros, in most cases in a dose-related manner. Consistent with this observation, splenic and pronephric hypocellularity was confirmed upon histological examination of chemical-treated fish. However, neither superoxide radical production or phagocytosis of splenic or pronephric macrophages was inhibited in either DMBA, lindane, or TCDD exposed fish. Results of this study indicate that depressed total cell number in fish hematopoietic organs may be a more sensitive indicator of exposure to these environmental contaminants than is the activity of macrophages contained within these organs. Master of Science 2014-03-14T21:45:59Z 2014-03-14T21:45:59Z 1996-07-15 2008-09-18 2008-09-18 2008-09-18 Thesis Text etd-09182008-063320 http://hdl.handle.net/10919/44809 http://scholar.lib.vt.edu/theses/available/etd-09182008-063320/ en OCLC# 35652800 LD5655.V855_1996.H378.pdf In Copyright http://rightsstatements.org/vocab/InC/1.0/ ix, 138 leaves BTD application/pdf application/pdf Virginia Tech
collection NDLTD
language en
format Others
sources NDLTD
topic flow cytometry
histology
immunosuppression
fish hematopoietic organs
macrophage
LD5655.V855 1996.H378
spellingShingle flow cytometry
histology
immunosuppression
fish hematopoietic organs
macrophage
LD5655.V855 1996.H378
Hart, Laura J.
Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)
description Alterations of immune parameters were investigated in fish exposed to non-overtly toxic levels of three different environmentally relevant chemicals, 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8- tetrachlorodibenzo-p -dioxin (TCDD). Each chemical agent was administered to tilapia in separate experiments by intraperitoneal injection for five consecutive days. Following the final dose, total cellularity and histology of the spleen and pronephros were assessed, as were activity of phagocytic celis contained in these hematopoietic organs. <p>Using chemical doses which produced no clinical toxicity, tilapia exposed to each chemical agent displayed a significant reduction in total cell number of both spleen and pronephros, in most cases in a dose-related manner. Consistent with this observation, splenic and pronephric hypocellularity was confirmed upon histological examination of chemical-treated fish. However, neither superoxide radical production or phagocytosis of splenic or pronephric macrophages was inhibited in either DMBA, lindane, or TCDD exposed fish. Results of this study indicate that depressed total cell number in fish hematopoietic organs may be a more sensitive indicator of exposure to these environmental contaminants than is the activity of macrophages contained within these organs. === Master of Science
author2 Veterinary Medical Sciences
author_facet Veterinary Medical Sciences
Hart, Laura J.
author Hart, Laura J.
author_sort Hart, Laura J.
title Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)
title_short Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)
title_full Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)
title_fullStr Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)
title_full_unstemmed Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)
title_sort subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (dmba), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>oreochromis niloticus</i>)
publisher Virginia Tech
publishDate 2014
url http://hdl.handle.net/10919/44809
http://scholar.lib.vt.edu/theses/available/etd-09182008-063320/
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