Evaluation of a mitochondrial test for the determination of chemical toxicity
The feasibility of using rat liver mitochondria respiratory parameters as a short-term toxicity test was investigated. Mitochondrial fractions were exposed to six concentrations of five chlorophenols. Respiratory parameters were measured and compared to control experiments. The toxicity of the c...
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| Format: | Others |
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Virginia Tech
2014
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| Online Access: | http://hdl.handle.net/10919/43054 http://scholar.lib.vt.edu/theses/available/etd-06102012-040255/ |
| Summary: | The feasibility of using rat liver mitochondria respiratory parameters
as a short-term toxicity test was investigated. Mitochondrial fractions were
exposed to six concentrations of five chlorophenols. Respiratory parameters
were measured and compared to control experiments. The toxicity of the
chlorophenos, measured by the 50% uncoupling concentration (UC50),
increased with increasing chloro substitution.
The UC50 values for the five chlorophenols were compared to six
physicochemical parameters for the same chlorophenols and high degrees of
correlation were found (r >/- 0.890). The highest correlation coefficient
obtained was with the octanol-water partition coefficient. UC50 values were
also compared to nine currently existing short-term toxicity tests. High
degrees of correlation were obtained with several of these tests, including
bacterial and fish bioassays.
From the results of these experiments, the measurement and use of
mitochondria respiratory parameters as a short-term toxicity test appears to
offer an alternative to currently used short-term toxicity tests, particularly
with chemicals having physicochemical characteristics similar to
mitochondria uncouplers. === Master of Science |
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