Sanitizer efficacy towards attached bacteria in a simulated milk pipeline system using pure and mixed cultures
The efficacy of six sanitizers, [chloline (200 ppm), iodophor (2S: ppm), acid anionic (200 ppm), peracetic acid (200 ppm), and fatty acid sanitizer (200 ppm)], was evaluated against bacteria attached to gasket materials. Pseudomonas fluorescens, Yersinia enterocolitica, Bacillus cereus, and Liste...
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| Format: | Others |
| Language: | en |
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Virginia Tech
2014
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| Online Access: | http://hdl.handle.net/10919/39061 http://scholar.lib.vt.edu/theses/available/etd-08062007-094410/ |
| Summary: | The efficacy of six sanitizers, [chloline (200 ppm), iodophor (2S: ppm),
acid anionic (200 ppm), peracetic acid (200 ppm), and fatty acid sanitizer (200
ppm)], was evaluated against bacteria attached to gasket materials. Pseudomonas
fluorescens, Yersinia enterocolitica, Bacillus cereus, and Listeria monocvtogenes
were capable of significant attachment to both buna-N nlbber and Teflon® gasket
surfaces in either pure or mixed cultures. Differences in initial attachment rates
were evident in a mixed culture of P. fluorescens, Y. enterocolitica, and Listeria
monocytogenes in vitro. Sanitizer effectiveness depended upon the bacterium
being enumerated, the type of surface, if the bacterium was attached in pure
culture or as part of a mixed culture, and the system of evaluation, (i.e. whether
or not sanitizer was used alone or as part of a cleaning system). Peracetic acid was
the most effective. Removal of bacteria was more pronounced on the Te'f1on®
surface with all sanitizers used. The cleaning system, which consisted of a pre-rinse with warm water, application of the cleaning solution, post-rinse with warm water,
and application of the sanitizing solution, allowed microorganisnls to rernain, when
the bacteria were present as a pure culturej but, resulted in the complete removal
of bacteria in mixed culture. === Ph. D. |
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