Carbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism
Carbonic anhydrase (CA) from acetate-grown Methanosarcina thermophila strain TM-1 was purified> 10,OOO-fold (22% recovery) to apparent homogeneity and a specific activity of 4,900 units mg⁻¹.The gene encoding this CA was isolated fronl a partial genomic library on a 12-kb fragment and sequenced....
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Format: | Others |
Language: | en |
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Virginia Tech
2014
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Online Access: | http://hdl.handle.net/10919/38486 http://scholar.lib.vt.edu/theses/available/etd-06062008-171625/ |