DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS

I describe a methodology for preparing and using a standard target for calibrating, laser alignment and focusing of MALDI imaging mass spectrometry instruments. The target is constructed by simple detachment patterning of a sublimed rubrene layer on an ITO glass slide. This pattern enables the measu...

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Main Author:	Rawhouser, Patrick Dale
Other Authors:	Richard Caprioli
Format:	Others
Language:	en
Published:	VANDERBILT 2012
Subjects:	Chemistry
Online Access:	http://etd.library.vanderbilt.edu/available/etd-12122012-141150/

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spelling	ndltd-VANDERBILT-oai-VANDERBILTETD-etd-12122012-1411502013-01-08T17:17:06Z DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS Rawhouser, Patrick Dale Chemistry I describe a methodology for preparing and using a standard target for calibrating, laser alignment and focusing of MALDI imaging mass spectrometry instruments. The target is constructed by simple detachment patterning of a sublimed rubrene layer on an ITO glass slide. This pattern enables the measurement of the effective laser spot size as well as the accuracy of the raster step size at scale of 2 µm and larger for selected instrument settings. The use of the target is demonstrated on two MALDI TOF instruments, a highly modified Applied Biosystems 4700 instrument with an effective laser spot size of 2.5 µm and a commercial UltrafleXtreme with a laser spot size of 20-40 µm. Richard Caprioli VANDERBILT 2012-12-14 text application/pdf http://etd.library.vanderbilt.edu/available/etd-12122012-141150/ http://etd.library.vanderbilt.edu/available/etd-12122012-141150/ en unrestricted I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.
collection	NDLTD
language	en
format	Others
sources	NDLTD
topic	Chemistry
spellingShingle	Chemistry Rawhouser, Patrick Dale DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS
description	I describe a methodology for preparing and using a standard target for calibrating, laser alignment and focusing of MALDI imaging mass spectrometry instruments. The target is constructed by simple detachment patterning of a sublimed rubrene layer on an ITO glass slide. This pattern enables the measurement of the effective laser spot size as well as the accuracy of the raster step size at scale of 2 µm and larger for selected instrument settings. The use of the target is demonstrated on two MALDI TOF instruments, a highly modified Applied Biosystems 4700 instrument with an effective laser spot size of 2.5 µm and a commercial UltrafleXtreme with a laser spot size of 20-40 µm.
author2	Richard Caprioli
author_facet	Richard Caprioli Rawhouser, Patrick Dale
author	Rawhouser, Patrick Dale
author_sort	Rawhouser, Patrick Dale
title	DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS
title_short	DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS
title_full	DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS
title_fullStr	DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS
title_full_unstemmed	DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS
title_sort	development of a standard reticle target for calibration of maldi imaging mass spectrometry instruments
publisher	VANDERBILT
publishDate	2012
url	http://etd.library.vanderbilt.edu/available/etd-12122012-141150/
work_keys_str_mv	AT rawhouserpatrickdale developmentofastandardreticletargetforcalibrationofmaldiimagingmassspectrometryinstruments
_version_	1716570644506738688

DEVELOPMENT OF A STANDARD RETICLE TARGET FOR CALIBRATION OF MALDI IMAGING MASS SPECTROMETRY INSTRUMENTS

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