African Swine Fever Virus DNA polymerase X: biophysical interaction studies and NMR assignments of the polymerase-deoxyguanosine triphosphate complex.

Three constructs of African Swine Fever Virus DNA polymerase X (pol X) were expressed and purified. Buffers containing sodium acetate, sodium cacodylate or 0.5 M NaCl provided good solubility for the enzyme at micromolar concentration, which was required for biophysical studies. The transformation b...

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Bibliographic Details
Main Author: Voehler, Markus Wolfgang
Other Authors: Brian O. Bachmann
Format: Others
Language:en
Published: VANDERBILT 2007
Subjects:
Online Access:http://etd.library.vanderbilt.edu/available/etd-12062007-172925/

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