African Swine Fever Virus DNA polymerase X: biophysical interaction studies and NMR assignments of the polymerase-deoxyguanosine triphosphate complex.
Three constructs of African Swine Fever Virus DNA polymerase X (pol X) were expressed and purified. Buffers containing sodium acetate, sodium cacodylate or 0.5 M NaCl provided good solubility for the enzyme at micromolar concentration, which was required for biophysical studies. The transformation b...
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| Format: | Others |
| Language: | en |
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VANDERBILT
2007
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| Online Access: | http://etd.library.vanderbilt.edu/available/etd-12062007-172925/ |