HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET

HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET

<p>Mental health problems related to dysfunction of serotonin transporter protein and norepinephrine transporter protein are an ever increasing burden on the population and the health care system. The Rosenthal lab uses a unique system that employs small molecule targeting ligands and quantum...

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Bibliographic Details
Main Author: Hughes, Emma Bronwyn
Other Authors: Dr. Sandra Rosenthal
Format: Others
Language:en
Published: VANDERBILT 2012
Subjects:
Chemistry
Online Access:http://etd.library.vanderbilt.edu/available/etd-04022012-134344/
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spelling ndltd-VANDERBILT-oai-VANDERBILTETD-etd-04022012-1343442013-01-08T17:16:58Z HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET Hughes, Emma Bronwyn Chemistry <p>Mental health problems related to dysfunction of serotonin transporter protein and norepinephrine transporter protein are an ever increasing burden on the population and the health care system. The Rosenthal lab uses a unique system that employs small molecule targeting ligands and quantum dots to image SERT and NET in live cells. The movement of the transporter proteins can be imaged in real time for much longer than with traditional flurophores due to the unique properties of the quantum dots. This project focused on testing new targeting ligands. The SERT targeting ligand IDT361 showed the ability to label SERT with quantum dots in live Hek293T cells. NET targeting ligand IDT545 was tested in live cells but did show high enough specificity to deter the uptake ability of NET in live cells and therefore is unlikely to be used in further study. Dr. Sandra Rosenthal Dr. Eva Harth Dr. David Wright VANDERBILT 2012-04-19 text application/pdf http://etd.library.vanderbilt.edu/available/etd-04022012-134344/ http://etd.library.vanderbilt.edu/available/etd-04022012-134344/ en unrestricted I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.
collection NDLTD
language en
format Others
sources NDLTD
topic Chemistry
spellingShingle Chemistry
Hughes, Emma Bronwyn
HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET
description <p>Mental health problems related to dysfunction of serotonin transporter protein and norepinephrine transporter protein are an ever increasing burden on the population and the health care system. The Rosenthal lab uses a unique system that employs small molecule targeting ligands and quantum dots to image SERT and NET in live cells. The movement of the transporter proteins can be imaged in real time for much longer than with traditional flurophores due to the unique properties of the quantum dots. This project focused on testing new targeting ligands. The SERT targeting ligand IDT361 showed the ability to label SERT with quantum dots in live Hek293T cells. NET targeting ligand IDT545 was tested in live cells but did show high enough specificity to deter the uptake ability of NET in live cells and therefore is unlikely to be used in further study.
author2 Dr. Sandra Rosenthal
author_facet Dr. Sandra Rosenthal
Hughes, Emma Bronwyn
author Hughes, Emma Bronwyn
author_sort Hughes, Emma Bronwyn
title HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET
title_short HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET
title_full HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET
title_fullStr HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET
title_full_unstemmed HIGH AFFINITY LIGANDS FOR THE TARGETING OF SERT AND NET
title_sort high affinity ligands for the targeting of sert and net
publisher VANDERBILT
publishDate 2012
url http://etd.library.vanderbilt.edu/available/etd-04022012-134344/
work_keys_str_mv AT hughesemmabronwyn highaffinityligandsforthetargetingofsertandnet
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