Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance

In this thesis, the structural differences between wild-type band 3 and band 3 Fukuoka, the G130R mutant identified in cases of hereditary spherocytosis associated with reduction of protein 4.2, are examined. The G130R mutation occurs on the solvent-facing side of helix 2 on the globular portion of...

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Main Author:	Nathaniel, Elizabeth Anne Nalani
Other Authors:	Al Beth
Format:	Others
Language:	en
Published:	VANDERBILT 2010
Subjects:	Chemical and Physical Biology
Online Access:	http://etd.library.vanderbilt.edu/available/etd-03242010-175917/

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spelling	ndltd-VANDERBILT-oai-VANDERBILTETD-etd-03242010-1759172013-01-08T17:16:39Z Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Nathaniel, Elizabeth Anne Nalani Chemical and Physical Biology In this thesis, the structural differences between wild-type band 3 and band 3 Fukuoka, the G130R mutant identified in cases of hereditary spherocytosis associated with reduction of protein 4.2, are examined. The G130R mutation occurs on the solvent-facing side of helix 2 on the globular portion of the cytosolic domain of band 3. Site-directed spin labeling coupled with electron paramagnetic resonance techniques are presented as methods for investigating subtle changes to protein structure. While the overall structure of the band 3 dimer shows little change, side chain mobilities are altered on helix 2. Solvent accessibility, on the other hand, shows that helix 2 maintains its backbone structure and relative orientation on the surface of band 3. I believe these small changes to helix 2 affect the binding of band 3 to ankyrin due to the proximity to their interaction site. Al Beth Charles Cobb Hassane Mchaourab VANDERBILT 2010-04-30 text application/pdf http://etd.library.vanderbilt.edu/available/etd-03242010-175917/ http://etd.library.vanderbilt.edu/available/etd-03242010-175917/ en unrestricted I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.
collection	NDLTD
language	en
format	Others
sources	NDLTD
topic	Chemical and Physical Biology
spellingShingle	Chemical and Physical Biology Nathaniel, Elizabeth Anne Nalani Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance
description	In this thesis, the structural differences between wild-type band 3 and band 3 Fukuoka, the G130R mutant identified in cases of hereditary spherocytosis associated with reduction of protein 4.2, are examined. The G130R mutation occurs on the solvent-facing side of helix 2 on the globular portion of the cytosolic domain of band 3. Site-directed spin labeling coupled with electron paramagnetic resonance techniques are presented as methods for investigating subtle changes to protein structure. While the overall structure of the band 3 dimer shows little change, side chain mobilities are altered on helix 2. Solvent accessibility, on the other hand, shows that helix 2 maintains its backbone structure and relative orientation on the surface of band 3. I believe these small changes to helix 2 affect the binding of band 3 to ankyrin due to the proximity to their interaction site.
author2	Al Beth
author_facet	Al Beth Nathaniel, Elizabeth Anne Nalani
author	Nathaniel, Elizabeth Anne Nalani
author_sort	Nathaniel, Elizabeth Anne Nalani
title	Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance
title_short	Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance
title_full	Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance
title_fullStr	Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance
title_full_unstemmed	Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance
title_sort	characterization of band 3 variant g130r using site-directed spin labeling and electron paramagnetic resonance
publisher	VANDERBILT
publishDate	2010
url	http://etd.library.vanderbilt.edu/available/etd-03242010-175917/
work_keys_str_mv	AT nathanielelizabethannenalani characterizationofband3variantg130rusingsitedirectedspinlabelingandelectronparamagneticresonance
_version_	1716533302018441216

Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance

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