Spider Aggregate Glue Sequence Characterization and Expression

Spider Aggregate Glue Sequence Characterization and Expression

Spider aggregate glue is secreted on to the webs of many spiders in the superfamily Araneoidea. Aggregate glue is sticky when wet and has a unique stress strain curve that varies depending on the relative humidity and speed of probe retraction. These features make it an attractive target for applica...

Full description

Bibliographic Details
Main Author: Berg, Kyle
Format: Others
Published: DigitalCommons@USU 2016
Subjects:
aggregate
spider
glue
adhesive
protein
Biology
Online Access:https://digitalcommons.usu.edu/etd/5022
https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=6060&context=etd
id ndltd-UTAHS-oai-digitalcommons.usu.edu-etd-6060
record_format oai_dc
spelling ndltd-UTAHS-oai-digitalcommons.usu.edu-etd-60602019-10-13T05:52:29Z Spider Aggregate Glue Sequence Characterization and Expression Berg, Kyle Spider aggregate glue is secreted on to the webs of many spiders in the superfamily Araneoidea. Aggregate glue is sticky when wet and has a unique stress strain curve that varies depending on the relative humidity and speed of probe retraction. These features make it an attractive target for applications such as underwater adhesives or surgical glues. However, little is known about the genetic sequence of the glue protein itself. In this work, a method is identified to isolate the remainder of the aggregate gene sequence, and genetic constructs glue are created using known aggregate gene sequence. One construct is expressed in E. coli BL21 cells and the protein is tested for its adhesive properties. 2016-05-01T07:00:00Z text application/pdf https://digitalcommons.usu.edu/etd/5022 https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=6060&context=etd Copyright for this work is held by the author. Transmission or reproduction of materials protected by copyright beyond that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user. For more information contact Andrew Wesolek (andrew.wesolek@usu.edu). All Graduate Theses and Dissertations DigitalCommons@USU aggregate spider glue adhesive protein Biology
collection NDLTD
format Others
sources NDLTD
topic aggregate
spider
glue
adhesive
protein
Biology
spellingShingle aggregate
spider
glue
adhesive
protein
Biology
Berg, Kyle
Spider Aggregate Glue Sequence Characterization and Expression
description Spider aggregate glue is secreted on to the webs of many spiders in the superfamily Araneoidea. Aggregate glue is sticky when wet and has a unique stress strain curve that varies depending on the relative humidity and speed of probe retraction. These features make it an attractive target for applications such as underwater adhesives or surgical glues. However, little is known about the genetic sequence of the glue protein itself. In this work, a method is identified to isolate the remainder of the aggregate gene sequence, and genetic constructs glue are created using known aggregate gene sequence. One construct is expressed in E. coli BL21 cells and the protein is tested for its adhesive properties.
author Berg, Kyle
author_facet Berg, Kyle
author_sort Berg, Kyle
title Spider Aggregate Glue Sequence Characterization and Expression
title_short Spider Aggregate Glue Sequence Characterization and Expression
title_full Spider Aggregate Glue Sequence Characterization and Expression
title_fullStr Spider Aggregate Glue Sequence Characterization and Expression
title_full_unstemmed Spider Aggregate Glue Sequence Characterization and Expression
title_sort spider aggregate glue sequence characterization and expression
publisher DigitalCommons@USU
publishDate 2016
url https://digitalcommons.usu.edu/etd/5022
https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=6060&context=etd
work_keys_str_mv AT bergkyle spideraggregategluesequencecharacterizationandexpression
_version_ 1719266914860007424

Similar Items