Detailed Description and Nomenclature of High Resolution G-Banded Horse Chromosomes and Cytogenetic Analysis of Hereditary Multiple Exostosis in Horses

• Main Author: Maciulis, Alma
• Format: Others
• Published: DigitalCommons@USU 1984
• Subjects: Animal Sciences; Dairy Science; Veterinary Medicine
• Online Access: https://digitalcommons.usu.edu/etd/4157; https://digitalcommons.usu.edu/cgi/viewcontent.cgi?article=5169&context=etd

A reliable technique for obtaining late prophase, prometaphase and greater numbers of metaphase chromosomes is presented. The increased number of mitoses and high resolution chromosomes were obtained using a modification of the typical three-day blood c ulture technique in which the cell cycle was temporarily blocked at the G1 -s border with methotrexate. A high resolution G-band idiogram is presented using a system similar to the standardization of human chromosomes. The use of this technique and system of nomenclature will facilitate routine analyses of horse chromosomes and provide a basis for the identification and recording of chromosome aberrations in this species. C-bands and NORs of the domestic horse are also described. C-bands, NORs and high resolution G-bands of control horses were compared with those of horses affected with Hereditary Multiple Exostosis. No differences were found on NORs, C- and G-bands between control and exostosis horses.