Nanoparticles’ effect in an in vitro whole blood model

• Main Author: Korkis, Layal
• Format: Others
• Language: English
• Published: Uppsala universitet, Institutionen för immunologi, genetik och patologi 2019
• Subjects: Silica; Titanium dioxide; Palladium; complement system; contact system.; Immunology in the medical area; Immunologi inom det medicinska området; Biomedical Laboratory Science/Technology; Biomedicinsk laboratorievetenskap/teknologi
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-392201

Nanoparticles have been used in industry and in medicine due to their properties which give them beneficial uses. This usage of the nanoparticles has risen the question about how harmful they are to the human body, the connection between the exposure to nanoparticles, and many diseases that occur in the body. Methods This study focused on the effect of nanoparticles in a whole human blood loop model. The blood was incubated with Silica, Titanium dioxide and Palladium particles in heparinized loops without any anticoagulants added. The blood’s cell count was analyzed with a cell counter and then complement, and contact system’s markers were analyzed with ELISA to detect a presence of activations in the systems. Experiments one to five were an optimization of test settings. Results An activation of the contact system was initiated in the loops containing the aggregated titanium dioxide nanoparticles. A high platelets consumption up to 73.8 % was observed as well as two visible clots. On top of that, blood smears showed micro-clots in the blood incubated with the aggregated nanoparticles. Conclusion Nanoparticles initiated an activation in the contact system in the aggregated form in comparison with the dispersed form. Further and deeper studies should be executed to observe the importance of the single or the aggregated form in the actual effect on the immune system.