General image classifier for fluorescence microscopy using transfer learning

• Main Author: Öhrn, Håkan
• Format: Others
• Language: English
• Published: Uppsala universitet, Avdelningen för visuell information och interaktion 2019
• Subjects: transfer learning; conformal prediction; image classification; Computer Sciences; Datavetenskap (datalogi)
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-388633

Modern microscopy and automation technologies enable experiments which can produce millions of images each day. The valuable information is often sparse, and requires clever methods to find useful data. In this thesis a general image classification tool for fluorescence microscopy images was developed usingfeatures extracted from a general Convolutional Neural Network (CNN) trained on natural images. The user selects interesting regions in a microscopy image and then, through an iterative process, using active learning, continually builds a training data set to train a classifier that finds similar regions in other images. The classifier uses conformal prediction to find samples that, if labeled, would most improve the learned model as well as specifying the frequency of errors the classifier commits. The result show that with the appropriate choice of significance one can reach a high confidence in true positive. The active learning approach increased the precision with a downside of finding fewer examples.