Antibiotic free and optimised protein production using Escherichia coli

• Main Authors: Engström, Mathias, Pontén, Olle, Philip, Carlsson, Bahnam, Nadeen, Strömberg, Ella, Westlin, Oskar
• Format: Others
• Language: English
• Published: Uppsala universitet, Institutionen för biologisk grundutbildning 2018
• Subjects: TAT; recombinant protein production; optimisation; fermentation; chromatography; protein purification; filtration; excretion system; affibody; biopharmaceuticals; validation; Industrial Biotechnology; Industriell bioteknik
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-352528

Affibody® molecules are small therapeutic proteins which mimics antibody functionality. This is a report of several methods for increasing productivity and yield in recombinant production of Affibody® molecules. This literature study shows several steps in the production line which can be optimised, several novel methods for cultivating and harvesting cells and purication of proteins. There is also a section about validation of therapeutic protein production according to The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) are presented.