TaqMan® Sample-to-SNP Kit™ : evaluation of kit for low-cost and fast preparing of DNA-samples before genotype analysis

• Main Author: Andersson, Eva
• Format: Others
• Language: Swedish
• Published: Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi 2009
• Subjects: Allelic discrimination; single nucleotide polymorphism; DNA extraction kit; blood; real-time PCR; Clinical chemistry; Klinisk kemi
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-105963

  Genotyping can be used to link genetic variation among individuals to certain diseases or conditions. Some known disorders and states that are dependent on single nucleotide polymorphism (SNPs) are lactose intolerance, venous thrombosis, hereditary hemochromatosis and the difference in sensibility among people to metabolise drugs. In this project a new kit, TaqManÒ Sample-to-SNP KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method. The purpose of the comparison was to find a method that makes DNA extraction from blood samples cheaper and faster, but with the same reliability as the reference procedure. The results of the evaluation showed a complete agreement of the genotype results between the methods tested, which means that the new method was as reliable as the reference method. The costs of reagents and material would be reduced with 52% if the new method is adopted, that alone would result in a cost reduction of 144 000SEK a year with a sample volume of 650 samples/month. The time for DNA extraction would also be reduced with the new procedure.