EN JÄMFÖRELSESTUDIE AV PLAST- OCH GLASHEMOCYTOMETRAR FÖR BERÄKNING AV BLODCELLER I VENÖST OCH KAPILLÄRT HELBLOD

• Main Author: Istrefi, Linda
• Format: Others
• Language: Swedish
• Published: Malmö högskola, Fakulteten för hälsa och samhälle (HS) 2015
• Subjects: Capillary whole blood; Hemocytometer; Platelet count; Sysmex XN-2000; White blood cell count; Medical and Health Sciences; Medicin och hälsovetenskap
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-26966

Hematology-instruments that analyze blood cells can at specific casesbe followed by manual counting with a hemocytometer. The plastic hemocytometer which has a fixed coverslip and is assigned for disposable use, appears to reduce the problems arising from the use of the traditional glass hemocytometer, for instance coverslip-application difficulty and dust particles. Capillary whole blood sampling may be useful for patients at the emergency department and in addition, a smaller volume of blood is taken compared to venous blood sampling. This study will focus on the platelet- and leukocyte count (PLT and WBC) in venous and capillary blood, in order to compare the plastic- and glass hemocytometers with Sysmex XN-2000 (Sysmex Corporation, Kobe, Japan), and verify if capillary whole blood sampling can be used for the measurement of these hematological parameters. 30 subjects donated both capillary- and venous blood samples and these blood samples were analyzed with glass- and plastic hemocytometers with Sysmex XN-2000 as the reference method. The study results showed high correlation between the reference method and plastic hemocytometer at low PLT but were not suitable for WBC-determination. The capillary whole blood showed high correlation to the reference method at WBC-determination, but was unusable at PLT-determination. Glass hemocytometer was closer to the reference method results than plastic hemocytometer was.