Luftprovtagning samt analys av mono- och diisocyanater

• Main Author: Komorowska, Marta
• Format: Others
• Language: Swedish
• Published: Linköpings universitet, Institutionen för fysik, kemi och biologi 2012
• Subjects: Isocyanater; impinger; luftprovtagning; torr provtagning; LC-MS/MS
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-85247

When exposed to them, isocyanates can induce serious injuries in the respiratory tract and irritation on the skin and in the eyes. They are therefore interesting from the point of view of occupational health. The purpose of this thesis was to collect isocyanates in air with impinger-filter samplers and solvent free samplers. Furthermore the isocyanates were to be analyzed with liquid chromatography-mass spectrometry. The solvent free sampler consists of a polypropylene tube and filter holder fitted with glass fiber filters impregnated with derivatization reagent, coupled with a pump. The impinger-filter sampler was made out of an impinger flask, containing a derivatization solution, coupled in series with a filter holder and a pump. Di-n-butylamine was used as derivatization reagent in both samplers to stabilize the reactive isocyanates and to enable mass spectrometric detection. The solvent free sampler is highly advantageous because of its user friendliness during field measurements, as opposed to the impinger-filter method. An exposure chamber, equipped with two interior fans to ensure good circulation, was used to generate an atmosphere containing isocyanates. Analyzing the isocyanate-DBA derivates with LC-MS/MS worked very well and the method made it possible to detect isocyanate levels below 1 ng/mL. During quantification of isocyanates a standard curve with concentrations from 1 to 1000 ng/mL was used. Detection of isocyanate levels as low as one fifth of the limit value of some isocyanates was found to be possible, which would indicate that the methods are sensitive. Even though the solvent free sampler worked, the impinger-filter sampler was found to be more effective in collecting isocyanates. The coefficient of variation calculated from concentrations of isocyanates from the solvent free sampler varied between 0-35 %. The reason for this might be due to the fact that an optimized extraction method had not been tried out within the time limit of this project. Questions, identified during this thesis work, need to be answered before being able to obtain reliable results from field measurements with the solvent free sampler.