MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles

There is an increasing interest for nanomaterials in bio-medical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3 ) have been investigated as a novel contrast agent for magnetic resonance imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in...

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Main Author: Hedlund, Anna
Format: Doctoral Thesis
Language:English
Published: Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV 2011
Subjects:
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-68802
http://nbn-resolving.de/urn:isbn:978-91-7393-215-8
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spelling ndltd-UPSALLA1-oai-DiVA.org-liu-688022013-09-06T04:01:54ZMRI Contrast Enhancement and Cell Labeling using Gd2O3 NanoparticlesengHedlund, AnnaLinköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIVLinköpings universitet, Medicinsk radiologiLinköpings universitet, HälsouniversitetetLinköping : Linköping University Electronic Press2011MEDICINEMEDICINThere is an increasing interest for nanomaterials in bio-medical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3 ) have been investigated as a novel contrast agent for magnetic resonance imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in cell culture medium and the nanoparticles have been explored as cell labeling agents. The fluorescent properties of the particles were used to visualize the internalization in cells and doped particles were investigated as a multimodal agent that could work as a fluorescent marker for microscopy and as a contrast enhancer for MRI. Fluorescent studies show that the Gd2O3 nanoparticles doped with 5% terbium have interesting fluorescent properties and that these particles could work as such multimodal contrast agent. Relaxivity measurements show that in aqueous solutions, there is a twofold increase in relaxivity for Gd2O3 compared to commercial agent Gd-DTPA. In cell culture medium as well as in cells, there is a clear T1 effect and an increase in signal intensity in T1-mapped images. The cellular uptake of Gd2O3 nanoparticles were increased with the use of transfection agent protamine sulfate. This work shows that Gd2O3 nanoparticles possess good relaxation properties that are retained in different biological environments. Gd2O3 particles are suitable as a T1 contrast agent, but seem also be adequate for T2 enhancement in forinstance cell labeling experiments. Doctoral thesis, comprehensive summaryinfo:eu-repo/semantics/doctoralThesistexthttp://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-68802urn:isbn:978-91-7393-215-8Linköping University Medical Dissertations, 0345-0082 ; 1230application/pdfinfo:eu-repo/semantics/openAccess
collection NDLTD
language English
format Doctoral Thesis
sources NDLTD
topic MEDICINE
MEDICIN
spellingShingle MEDICINE
MEDICIN
Hedlund, Anna
MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
description There is an increasing interest for nanomaterials in bio-medical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3 ) have been investigated as a novel contrast agent for magnetic resonance imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in cell culture medium and the nanoparticles have been explored as cell labeling agents. The fluorescent properties of the particles were used to visualize the internalization in cells and doped particles were investigated as a multimodal agent that could work as a fluorescent marker for microscopy and as a contrast enhancer for MRI. Fluorescent studies show that the Gd2O3 nanoparticles doped with 5% terbium have interesting fluorescent properties and that these particles could work as such multimodal contrast agent. Relaxivity measurements show that in aqueous solutions, there is a twofold increase in relaxivity for Gd2O3 compared to commercial agent Gd-DTPA. In cell culture medium as well as in cells, there is a clear T1 effect and an increase in signal intensity in T1-mapped images. The cellular uptake of Gd2O3 nanoparticles were increased with the use of transfection agent protamine sulfate. This work shows that Gd2O3 nanoparticles possess good relaxation properties that are retained in different biological environments. Gd2O3 particles are suitable as a T1 contrast agent, but seem also be adequate for T2 enhancement in forinstance cell labeling experiments.
author Hedlund, Anna
author_facet Hedlund, Anna
author_sort Hedlund, Anna
title MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
title_short MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
title_full MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
title_fullStr MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
title_full_unstemmed MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
title_sort mri contrast enhancement and cell labeling using gd2o3 nanoparticles
publisher Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV
publishDate 2011
url http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-68802
http://nbn-resolving.de/urn:isbn:978-91-7393-215-8
work_keys_str_mv AT hedlundanna mricontrastenhancementandcelllabelingusinggd2o3nanoparticles
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