MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
There is an increasing interest for nanomaterials in bio-medical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3 ) have been investigated as a novel contrast agent for magnetic resonance imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in...
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Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV
2011
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ndltd-UPSALLA1-oai-DiVA.org-liu-688022013-09-06T04:01:54ZMRI Contrast Enhancement and Cell Labeling using Gd2O3 NanoparticlesengHedlund, AnnaLinköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIVLinköpings universitet, Medicinsk radiologiLinköpings universitet, HälsouniversitetetLinköping : Linköping University Electronic Press2011MEDICINEMEDICINThere is an increasing interest for nanomaterials in bio-medical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3 ) have been investigated as a novel contrast agent for magnetic resonance imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in cell culture medium and the nanoparticles have been explored as cell labeling agents. The fluorescent properties of the particles were used to visualize the internalization in cells and doped particles were investigated as a multimodal agent that could work as a fluorescent marker for microscopy and as a contrast enhancer for MRI. Fluorescent studies show that the Gd2O3 nanoparticles doped with 5% terbium have interesting fluorescent properties and that these particles could work as such multimodal contrast agent. Relaxivity measurements show that in aqueous solutions, there is a twofold increase in relaxivity for Gd2O3 compared to commercial agent Gd-DTPA. In cell culture medium as well as in cells, there is a clear T1 effect and an increase in signal intensity in T1-mapped images. The cellular uptake of Gd2O3 nanoparticles were increased with the use of transfection agent protamine sulfate. This work shows that Gd2O3 nanoparticles possess good relaxation properties that are retained in different biological environments. Gd2O3 particles are suitable as a T1 contrast agent, but seem also be adequate for T2 enhancement in forinstance cell labeling experiments. Doctoral thesis, comprehensive summaryinfo:eu-repo/semantics/doctoralThesistexthttp://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-68802urn:isbn:978-91-7393-215-8Linköping University Medical Dissertations, 0345-0082 ; 1230application/pdfinfo:eu-repo/semantics/openAccess |
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English |
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Doctoral Thesis |
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MEDICINE MEDICIN |
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MEDICINE MEDICIN Hedlund, Anna MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles |
description |
There is an increasing interest for nanomaterials in bio-medical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3 ) have been investigated as a novel contrast agent for magnetic resonance imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in cell culture medium and the nanoparticles have been explored as cell labeling agents. The fluorescent properties of the particles were used to visualize the internalization in cells and doped particles were investigated as a multimodal agent that could work as a fluorescent marker for microscopy and as a contrast enhancer for MRI. Fluorescent studies show that the Gd2O3 nanoparticles doped with 5% terbium have interesting fluorescent properties and that these particles could work as such multimodal contrast agent. Relaxivity measurements show that in aqueous solutions, there is a twofold increase in relaxivity for Gd2O3 compared to commercial agent Gd-DTPA. In cell culture medium as well as in cells, there is a clear T1 effect and an increase in signal intensity in T1-mapped images. The cellular uptake of Gd2O3 nanoparticles were increased with the use of transfection agent protamine sulfate. This work shows that Gd2O3 nanoparticles possess good relaxation properties that are retained in different biological environments. Gd2O3 particles are suitable as a T1 contrast agent, but seem also be adequate for T2 enhancement in forinstance cell labeling experiments. |
author |
Hedlund, Anna |
author_facet |
Hedlund, Anna |
author_sort |
Hedlund, Anna |
title |
MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles |
title_short |
MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles |
title_full |
MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles |
title_fullStr |
MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles |
title_full_unstemmed |
MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles |
title_sort |
mri contrast enhancement and cell labeling using gd2o3 nanoparticles |
publisher |
Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV |
publishDate |
2011 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-68802 http://nbn-resolving.de/urn:isbn:978-91-7393-215-8 |
work_keys_str_mv |
AT hedlundanna mricontrastenhancementandcelllabelingusinggd2o3nanoparticles |
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1716597209427869696 |