Double integrating spheres: A method for assessment of optical properties of biological tissues

• Main Author: Poppendieck, Wigand
• Format: Others
• Language: Swedish
• Published: Linköpings universitet, Institutionen för medicinsk teknik 2004
• Subjects: Interdisciplinary studies; Double integrating sphere; optical properties; absorption; scattering; Inverse Adding Doubling; TVÄRVETENSKAP; Social Sciences Interdisciplinary; Tvärvetenskapliga studier inom samhällsvetenskap
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-2701

The determination of the optical properties of biological tissue is an important issue in laser medicine. The optical properties define the tissue´s absorption and scattering behaviour, and can be expressed by quantities such as the albedo, the optical thickness and the anisotropy coefficient. During this project, a measurement system for the determination of the optical properties was built up. The system consists of a double integrating sphere set-up to perform the necessary reflection and transmission measurements, and a computer algorithm to calculate the optical properties from the measured data. This algorithm is called Inverse Adding Doubling method, and is based on a one-dimensional transport model. First measurements were conducted with the system, including measurements with phantom media (Intralipid-ink solutions) and with cartilage samples taken from the human knee joint. This work also includes an investigation about the preparation of tissue samples for optical measurements.