Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the...

Full description

Bibliographic Details
Main Author:	Piguet, Joachim
Format:	Doctoral Thesis
Language:	English
Published:	Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland 2010
Subjects:	fluorescence microscopy plasma membrane membrane proteins membrane proteins diffusion single-molecule imaging single-molecule tracking pentameric ligand-gated ion channels nicotinic acetylcholine receptor serotonin receptor synapse neuromuscular junction post-synaptic scaffold rapsyn myopathies fluorescent proteins Förster resonance energy transfer FRET imaging protein-protein interactions protein trafficking photo-activatable proteins
Online Access:	http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-178147

Similar Items

Over the rainbow: A practical guide for fluorescent protein selection in plant FRET experiments
by: Grégoire Denay, et al.
Published: (2019-12-01)

Illuminating amyloid fibrils: Fluorescence-based single-molecule approaches
by: Lauren J. Rice, et al.
Published: (2021-01-01)

The MX-Helix of Muscle nAChR Subunits Regulates Receptor Assembly and Surface Trafficking
by: Jolene Chang Rudell, et al.
Published: (2020-03-01)

Analysis of GPI-Anchored Receptor Distribution and Dynamics in Live Cells by Tag-mediated Enzymatic Labeling and FRET
by: Maria N. Balatskaya, et al.
Published: (2020-04-01)

Protein–lipid architecture of a cholinergic postsynaptic membrane
by: Nigel Unwin
Published: (2020-09-01)

Single-Molecule FRET Imaging of Virus Spike–Host Interactions
by: Maolin Lu
Published: (2021-02-01)

The Role of the M4 α-Helix in Lipid Sensing by a Pentameric Ligand-Gated Ion Channel
by: Hénault, Camille
Published: (2021)

The dimerization equilibrium of a ClC Cl−/H+ antiporter in lipid bilayers
by: Rahul Chadda, et al.
Published: (2016-08-01)

Deciphering the Mechanism of E. coli tat Protien Transport: Kinetic Substeps and Cargo Properties
by: Whitaker, Neal William 1982-
Published: (2013)

Conformational Switching in Bcl-xL: Enabling Non-Canonic Inhibition of Apoptosis Involves Multiple Intermediates and Lipid Interactions
by: Victor Vasquez-Montes, et al.
Published: (2020-02-01)

Computational refinement of spectroscopic FRET measurements
by: Alexander Kyrychenko, et al.
Published: (2017-06-01)

Experimental approaches for addressing fundamental biological questions in living, functioning cells with single molecule precision
by: Tchern Lenn, et al.
Published: (2012-01-01)

Lipid Composition Affects the Efficiency in the Functional Reconstitution of the Cytochrome <i>c</i> Oxidase
by: Katharina Gloria Hugentobler, et al.
Published: (2020-09-01)

Investigating cotranslational integration of a multi-spanning membrane protein into the endoplasmic reticulum membrane
by: Jongsma, Candice Gene
Published: (2010)

Single Proteins under the Microscope: Conformations, Dynamics and Medicinal Therapies
by: Liu, Baoxu
Published: (2013)

Single Proteins under the Microscope: Conformations, Dynamics and Medicinal Therapies
by: Liu, Baoxu
Published: (2013)

Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers
by: Marijn de Boer, et al.
Published: (2019-03-01)

NANOFLUIDIC SINGLE MOLECULE DETECTION (SMD) FOR PROTEIN DETECTION AND INTERACTION DYNAMICS STUDY
by: Jing, Nan
Published: (2010)

A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome
by: Guanglin Xing, et al.
Published: (2019-09-01)

Site-directed MT1-MMP trafficking and surface insertion regulate AChR clustering and remodeling at developing NMJs
by: Zora Chui-Kuen Chan, et al.
Published: (2020-03-01)

Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells
by: Sara Mareike Müller, et al.
Published: (2013-10-01)

Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns
by: Daniella Lucena, et al.
Published: (2018-09-01)

Aquaporin Protein-Protein Interactions
by: Jennifer Virginia Roche, et al.
Published: (2017-10-01)

Early Amyloidogenic Oligomerization Studied through Fluorescence Lifetime Correlation Spectroscopy
by: Angel Orte, et al.
Published: (2012-07-01)

Cadherin clusters stabilized by a combination of specific and nonspecific cis-interactions
by: Connor J Thompson, et al.
Published: (2020-09-01)

High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells
by: Hanieh Mazloom-Farsibaf, et al.
Published: (2020-06-01)

Cellular encoding of Cy dyes for single-molecule imaging
by: Lilia Leisle, et al.
Published: (2016-12-01)

Investigating cotranslational protein integration into the endoplasmic reticulum membrane
by: McCormick, Peter Joseph
Published: (2005)

Single-molecule force spectroscopy of protein-membrane interactions
by: Lu Ma, et al.
Published: (2017-10-01)

Single Molecule FRET: A Powerful Tool to Study Intrinsically Disordered Proteins
by: Sharonda J. LeBlanc, et al.
Published: (2018-11-01)

Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules
by: Max S. Fairlamb, et al.
Published: (2021-06-01)

Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation
by: Daniëlle Rianne José Verboogen, et al.
Published: (2017-05-01)

Two-step membrane binding by the bacterial SRP receptor enable efficient and accurate Co-translational protein targeting
by: Yu-Hsien Hwang Fu, et al.
Published: (2017-07-01)

Supra-Molecular Assemblies of ORAI1 at Rest Precede Local Accumulation into Puncta after Activation
by: Diana B. Peckys, et al.
Published: (2021-01-01)

Reconstitution of purified membrane protein dimers in lipid nanodiscs with defined stoichiometry and orientation using a split GFP tether
by: Bruguera, E.S, et al.
Published: (2022)

NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin
by: Ucheor B Choi, et al.
Published: (2018-07-01)

Receptor Quaternary Organization Explains G Protein-Coupled Receptor Family Structure
by: James H. Felce, et al.
Published: (2017-09-01)

Structural and biophysical characterization of the tandem substrate-binding domains of the ABC importer GlnPQ
by: Evelyn Ploetz, et al.
Published: (2021-04-01)

DNA-Mediated Stack Formation of Nanodiscs
by: Madhumalar Subramanian, et al.
Published: (2021-03-01)

Reciprocal modulation between amyloid precursor protein and synaptic membrane cholesterol revealed by live cell imaging
by: Claire E. DelBove, et al.
Published: (2019-07-01)