Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase

Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase

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<p>In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cya...

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Main Author: Mketsu, Moses Clive Masisange
Format: Others
Language:English
Published: 2009
Subjects:
Nitrogen compounds
Nitriles
Nitrogen.
Online Access:http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1166_1307514664
id ndltd-UNWC-oai-UWC_ETD-http%3A%2F%2Fetd.uwc.ac.za%2Findex.php%3Fmodule%3Detd%26action%3Dviewtitle%26id%3Dgen8Srv25Nme4_1166_1307514664
record_format oai_dc
spelling ndltd-UNWC-oai-UWC_ETD-http%3A%2F%2Fetd.uwc.ac.za%2Findex.php%3Fmodule%3Detd%26action%3Dviewtitle%26id%3Dgen8Srv25Nme4_1166_13075146642013-01-08T12:42:23Z Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase Mketsu, Moses Clive Masisange Nitrogen compounds Nitriles Nitrogen. <p>In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cyanopyridine as a substrate, whereas continuous enzyme kinetics were conducted using acrylonitrile as a substrate.</p> 2009 Thesis and dissertation Pdf http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1166_1307514664 English ZA Copyright: University of the Western Cape
collection NDLTD
language English
format Others
sources NDLTD
topic Nitrogen compounds
Nitriles
Nitrogen.
spellingShingle Nitrogen compounds
Nitriles
Nitrogen.
Mketsu, Moses Clive Masisange
Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
description <p>In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cyanopyridine as a substrate, whereas continuous enzyme kinetics were conducted using acrylonitrile as a substrate.</p>
author Mketsu, Moses Clive Masisange
author_facet Mketsu, Moses Clive Masisange
author_sort Mketsu, Moses Clive Masisange
title Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_short Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_full Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_fullStr Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_full_unstemmed Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
title_sort screening for subtate tolerant geobacillus pallidus rapc8 nitrile hydratase
publishDate 2009
url http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1166_1307514664
work_keys_str_mv AT mketsumosesclivemasisange screeningforsubtatetolerantgeobacilluspallidusrapc8nitrilehydratase
_version_ 1716506823027064832

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