Avaliação da influência da quimioterapia com cisplatina ou 5-fluorouracil sobre o processo de reparo ósseo em implantes osseointegrados instalados em tíbias de ratos expostos ou não à nicotina /

• Main Author: Matheus, Henrique Rinaldi.
• Other Authors: Universidade Estadual Paulista (Unesp) Faculdade de Odontologia
• Format: Others
• Language: Portuguese
• Published: Araçatuba, 2019
• Subjects: Nicotina.; Agentes antineoplasicos.; Implantes dentários.; Osseointegração.; Nicotine
• Online Access: http://hdl.handle.net/11449/180853

Orientador: Juliano Milanezi de Almeida === Banca: Edilson Ervolino === Banca: Estevam Augusto Bonfante === Resumo: Objetivo: avaliar a influência da nicotina e dos agentes antineoplásicos Cisplatina (CIS) e 5-fluorouracil (5-FU) sobre os tecidos peri-implantares, bem como os efeitos desses agentes antineoplásicos sobre os tecidos peri-implantares em animais previamente expostos à nicotina. Material e métodos: 180 ratos machos (Wistar) foram randomizados para dois grandes grupos (n=90), NIC e SS, em seguida, para três subgrupos (n=30) de acordo com os agentes antineoplásicos. Receberam 0,5ml de solução de cloreto de sódio 0,9% (SS) ou 3 mg/kg de hemissulfato de nicotina (NIC), de acordo com cada grupo, 30 dias antes e 30 dias após a cirurgia. No dia 0, todos os animais receberam os implantes de titânio (DSP Biomedical®, 4 mm x 2,2 mm) nas tíbias direita e esquerda. Aos 30 dias após a cirurgia, as aplicações de SS e NIC foram interrompidas por 5 dias e, aos 35 e 37 dias, foram administrados os agentes antineoplásicos CIS, 5-FU ou 0,5 ml de SS, via intraperitoneal, respeitando intervalo de 48 h entre as aplicações. Para CIS, 5 mg/kg e 2,5 mg/kg, e para 5-FU, 60 mg/kg e 40 mg/kg, respetivamente. SS-SS: receberam SS via subcutânea e intraperitoneal. SS-CIS: receberam SS via subcutânea e CIS via intraperitoneal. SS-5FU: receberam SS via subcutânea e 5-FU via intraperitoneal. NIC-SS: receberam NIC via subcutânea e SS via intraperitoneal. NIC-CIS: receberam NIC via subcutânea e CIS via intraperitoneal. NIC-5FU: receberam NIC via subcutânea e 5-FU via intraperitoneal. Dez animais por grupo/período... (Resumo completo, clicar acesso eletrônico abaixo) === Abstract: Objective: to evaluate the influence of nicotine and the antineoplastic agents Cisplatin (CIS) and 5-fluorouracil (5-FU) over the peri-implant tissues, as well as the effects of these agents over peri-implant tissues in animals previously exposed to nicotine. Material and Methods: 180 male rats (Wistar) were initially randomized to two groups (n=90), NIC and SS. Then, to three subgroups (n=30) according to the protocol of antineoplastic agents. Received 0.5 ml of sodium chloride 0.9% (SS) or 3 mg/kg of nicotine hemissulfate (NIC) according to each group, subcutaneously, 30 days before and after surgical procedure for implants placement. At day 0, all animals received the titanium implants (DSP Biomedical®, 4 mm x 2.2 mm) in both right and left tibiae. At 30 days after surgery SS and NIC was interrupted, and at 35 and 37 days were intraperitoneally administered the antineoplastic agents CIS, 5FU or 0.5 ml SS, with 48 h interval between applications. For CIS, 5 mg/kg and 2,5 mg/kg, and 5-FU, 60 mg/kg and 40 mg/kg, respectively. SS-SS: received SS subcutaneously and intraperitoneally. SS-CIS: received SS subcutaneously and CIS intraperitoneally. SS-5FU: received SS subcutaneously and 5-FU intraperitoneally. NIC-SS: received NIC subcutaneously and SS intraperitoneally. NIC-CIS: received NIC subcutaneously and CIS intraperitoneally. NIC-5FU: received NIC subcutaneously and 5-FU intraperitoneally. Ten animals per group and period were euthanized at 50, 65 and 95 days after implants placement. The collected specimens were fixed in buffered formaldehyde solution 4% for 48h and assigned to ground section processing for analysis of bone/implant contact (BIC), or conventional histologic processing with demineralization and paraffin embedding for histometric analysis of percentage of newly-formed bone (PNFB) (Complete abstract electronic access below) === Mestre