Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics the transition state of a corresponding amide or ester hydrolysis reaction. We applied hybridoma tec...
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| Language: | ENG |
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ScholarWorks@UMass Amherst
2000
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| Online Access: | https://scholarworks.umass.edu/dissertations/AAI9960802 |