| Summary: | A competitive ligand equilibration adsorptive cathodic stripping voltammetry (CLEACS
V) technique using salicylaldoxime as the added ligand was developed for the detennination
of copper speciation in seawater. The CLE-ACSV technique has been shown to be applicable to
the determination of total copper. The depth profile at station AVHS-1 in the Western North
Pacific has been reproduced, and the concentration of copper in the standard reference material
NASS-4 was determined to be 3.4 ± 0.4 nM in excellent agreement with the certified value of
3.6 ± 0.2 nM.
The applicability of this technique to the measurement of copper speciation was inferred
from the ability to accurately determine the value of the overall stability constant for the
Cu(SA)₂ complex by calibration with EDTA. The measured value of β₂sa/cu²⁺ = 15.0 ± 0.2 in
excellent agreement with the only literature value of β^lci+ = 14.88 ± 0.3.
Cultures of the marine cyanobacteria Synechococcus PCC7002 produce a strong copperbinding
ligand when exposed to gradual increases in copper. This ligand as been shown to have
a log K^u, of 12.2, amongst the strongest copper complexing ligands investigated to date. === Science, Faculty of === Earth, Ocean and Atmospheric Sciences, Department of === Graduate
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